Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.144 extracted from

  • Nagai, K.; Ihara, Y.; Wada, Y.; Taniguchi, N.
    N-Glycosylation is requisite for the enzyme activity and Golgi retention of N-acetylglucosaminyltransferase III (1997), Glycobiology, 7, 769-776.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutants in COS-1 cells Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
N243Q site-directed mutagenesis of 1 N-glycosylation site, 40-50% activity compared to the wild-type, slightly increased Km-value for the donor substrate Rattus norvegicus
N243Q/N261Q site-directed mutagenesis of 2 N-glycosylation sites, 10% activity compared to the wild-type Rattus norvegicus
N243Q/N261Q/N399Q site-directed mutagenesis of all N-glycosylation sites, no remaining activity Rattus norvegicus
N243Q/N399Q site-directed mutagenesis of 2 N-glycosylation sites, highly reduced activity Rattus norvegicus
N261Q site-directed mutagenesis of 1 N-glycosylation site, 40-50% activity compared to the wild-type, slightly increased Km-value for the donor substrate Rattus norvegicus
N261Q/N399Q site-directed mutagenesis of 2 N-glycosylation sites, highly reduced activity Rattus norvegicus
N399Q site-directed mutagenesis of 1 N-glycosylation site, 70-80% activity compared to the wild-type Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
castanospermine activity is not affected, but the enzyme is not localized in the Golgi apparatus Rattus norvegicus
tunicamycin completely abolishes the enzyme activity due to deglycosylation of the enzyme, not localized in the Golgi apparatus Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum tunicamycin-treated and castanospermine-treated enzyme, not native enzyme Rattus norvegicus 5783
-
Golgi apparatus deglycosylated mutants are not localized in the Golgi apparatus Rattus norvegicus 5794
-
Golgi apparatus wild-type Rattus norvegicus 5794
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68100
-
x * 68100, wild-type enzyme, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein requires core glycosylation but not glucose trimming Rattus norvegicus
glycoprotein 3 N-glycosylation sites: Asn243, Asn261, Asn399, essential for activity, tunicamycin-treated enzyme, which is not glycosylated, shows no activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-glucosamine + (N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
Rattus norvegicus UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(N-acetyl-beta-D-glucosaminyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
?
UDP-N-acetyl-D-glucosamine + GlcNAc-beta-1,2-Man-alpha-1,6-(GlcNAc-beta-1,2-Man-alpha-1,3)-Man-beta-1,4-GlcNAc-beta-1,4-GlcNAc-2-aminopyridine
-
Rattus norvegicus UDP + GlcNAc-beta-1,2-Man-alpha-1,6-(GlcNAc-beta-1,2-Man-alpha-1,3)(GlcNAc-beta-1,4)-Man-beta-1,4-GlcNAc-beta-1,4-GlcNAc-2-aminopyridine
-
?

Subunits

Subunits Comment Organism
? x * 68100, wild-type enzyme, SDS-PAGE Rattus norvegicus

Synonyms

Synonyms Comment Organism
GnT-III
-
Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus