Literature summary for 2.4.1.143 extracted from

Kajiura, H.; Nakamura, Y.; Nishimura, M.; Ohashi, T.; Misaki, R.; Fujiyama, K.
Characterization of Bombyx mori N-acetylglucosaminyltransferase II splicing variants (2020), Biochem. Biophys. Res. Commun., 529, 404-410 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Sf9 insect cells	Bombyx mori

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
Golgi apparatus	-	Bombyx mori	5794	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	dependent on	Bombyx mori

Organism

Organism	UniProt	Comment	Textmining
Bombyx mori	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Co2+ affinity column chromatography	Bombyx mori

Source Tissue

Source Tissue	Comment	Organism	Textmining
larva	-	Bombyx mori	-
silk gland	-	Bombyx mori	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-GlcNAc + GlcNAcMan3GlcNAc2-pyridylamine	-	Bombyx mori	UDP + GlcNAc2Man3GlcNAc2-pyridylamine	-	?

Subunits

Subunits	Comment	Organism
?	x * about 70000, SDS-PAGE	Bombyx mori

Synonyms

Synonyms	Comment	Organism
GnTII	-	Bombyx mori
N-acetylglucosaminyltransferase II	-	Bombyx mori

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	40	-	Bombyx mori

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
5	50	splice variants GNTII-A, -B, -C, and -D show 70.3%, 81.9%, 44.4% and 91.9% relative activity after 1 h at 30, 50, 40 and 50Â°C, respectively	Bombyx mori

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	7	-	Bombyx mori

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Release 2023.1 (January 2023)