Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glucose 6-phosphate | allosteric activation of the wild-type enzyme, N-terminally truncated enzymes are not activated | Saccharum officinarum |
Cloned (Comment) | Organism |
---|---|
gene SoSPS1, recombinant expression of N-terminaly His6-tagged wild-type enzyme and truncated mutant enzymes lacking up to 171 residues of the N-terminal region in Escherichia coli strain BL21(DE3), recombinant expression of only the full-length wild-type enzyme with a His8-myc-His8 tag, a TEV cleavage site, and EGFP fusion in Spodoptera frugiperda Sf9 cells, the latter via baculovirus transfection | Saccharum officinarum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of N-terminally truncated enzymeslacking up to 171 amino acid residues in the 20 kDa N-terminal region, the mutants cannot be activated | Saccharum officinarum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | allosteric regulation by glucose 6-phosphate | Saccharum officinarum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Saccharum officinarum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + D-fructose 6-phosphate | Saccharum officinarum | - |
UDP + sucrose 6F-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharum officinarum | P93782 | gene SoSPS1 | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) and His8-myc-His8 tagged EGFP-enzyme from insect Sf9 cells by anion exchange and nickel affinity chromatography, followed by gel filtration. Purification of the full-length enzyme without His-tag by ion exchange and hydrophobic interaction chromatography, and gel filtration | Saccharum officinarum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Saccharum officinarum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + D-fructose 6-phosphate | - |
Saccharum officinarum | UDP + sucrose 6F-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | two forms of recombinant enzyme, form A of 100 kDa and form B of 120 kDa, in Escherichia coli. Form B might be enzymatically more active than form A | Saccharum officinarum |
Synonyms | Comment | Organism |
---|---|---|
SPS | - |
Saccharum officinarum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 27 | assay at | Saccharum officinarum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharum officinarum |
General Information | Comment | Organism |
---|---|---|
additional information | the N-terminal region of sugarcane sucrose phosphate synthase is not essential for the catalytic reaction itself, but is crucial for the allosteric regulation by glucose 6-phosphate and may function like a suppressor domain for the enzyme activity | Saccharum officinarum |