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Literature summary for 2.4.1.14 extracted from

  • Aleman, L.; Ortega, J.L.; Martinez-Grimes, M.; Seger, M.; Holguin, F.O.; Uribe, D.J.; Garcia-Ibilcieta, D.; Sengupta-Gopalan, C.
    Nodule-enhanced expression of a sucrose phosphate synthase gene member (MsSPSA) has a role in carbon and nitrogen metabolism in the nodules of alfalfa (Medicago sativa L.) (2010), Planta, 231, 233-244.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
D-glucose 6-phosphate 157-165% activation, the activation effect is reduced in plants infected with an N-fixation deficient Sinorhizobium meliloti strain Medicago sativa

Cloned(Commentary)

Cloned (Comment) Organism
SPSA and SPSB, DNA and amino acid sequence determination and analysis Medicago sativa

Inhibitors

Inhibitors Comment Organism Structure
phosphate 86.3% inhibition of the nodule isozyme at 5 mM, 15.7% and 25.9% inhibition of the leaf enzyme at 2 mM and 5 mM, respectively. The inhibitory effect is increased in plants infected with an N-fixation deficient Sinorhizobium meliloti strain Medicago sativa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Medicago sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + D-fructose 6-phosphate Medicago sativa isozyme show differences in allosteric regulation UDP + sucrose 6F-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Medicago sativa Q9AXK3 cv. Mesa, plants inoculated with Sinorhizobium meliloti, three different isoforms of SPS belonging to two dfferent SPS gene families, SPSA and SPSB, in Medicago sativa
-

Purification (Commentary)

Purification (Comment) Organism
native isozymes partially by anion exchange chromatography Medicago sativa

Source Tissue

Source Tissue Comment Organism Textmining
leaf high expression level of SPSB, leaves of plants inoculated with Sinorhizobium meliloti Medicago sativa
-
root nodule high expression level of SPSA, nodules of plants inoculated with Sinorhizobium meliloti Medicago sativa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0316
-
wild-type leaf isozyme from plants infected with an N-fixation deficient Sinorhizobium meliloti strain Medicago sativa
0.0322
-
wild-type leaf isozyme from plants infected with a wild-type Sinorhizobium meliloti strain Medicago sativa
0.0767
-
wild-type nodule isozyme from plants infected with an N-fixation deficient Sinorhizobium meliloti strain Medicago sativa
0.0861
-
wild-type nodule isozyme from plants infected with a wild-type Sinorhizobium meliloti strain Medicago sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + D-fructose 6-phosphate isozyme show differences in allosteric regulation Medicago sativa UDP + sucrose 6F-phosphate
-
?
UDP-glucose + D-fructose 6-phosphate optimal substrate concentrations are 10 mM for D-fructose 6-phosphate and 12 mM for UDP-glucose Medicago sativa UDP + sucrose 6F-phosphate
-
?

Synonyms

Synonyms Comment Organism
More three dfferent isoforms of SPS belonging to two dfferent SPS gene families, SPSA and SPSB in Medicago sativa Medicago sativa
SPS
-
Medicago sativa
sucrose phosphate synthase
-
Medicago sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Medicago sativa

General Information

General Information Comment Organism
metabolism SPS catalyzes the first step in the synthesis of sucrose in photosynthetic tissue Medicago sativa