Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-glucose 6-phosphate | 157-165% activation, the activation effect is reduced in plants infected with an N-fixation deficient Sinorhizobium meliloti strain | Medicago sativa |
Cloned (Comment) | Organism |
---|---|
SPSA and SPSB, DNA and amino acid sequence determination and analysis | Medicago sativa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | 86.3% inhibition of the nodule isozyme at 5 mM, 15.7% and 25.9% inhibition of the leaf enzyme at 2 mM and 5 mM, respectively. The inhibitory effect is increased in plants infected with an N-fixation deficient Sinorhizobium meliloti strain | Medicago sativa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Medicago sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + D-fructose 6-phosphate | Medicago sativa | isozyme show differences in allosteric regulation | UDP + sucrose 6F-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Medicago sativa | Q9AXK3 | cv. Mesa, plants inoculated with Sinorhizobium meliloti, three different isoforms of SPS belonging to two dfferent SPS gene families, SPSA and SPSB, in Medicago sativa | - |
Purification (Comment) | Organism |
---|---|
native isozymes partially by anion exchange chromatography | Medicago sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | high expression level of SPSB, leaves of plants inoculated with Sinorhizobium meliloti | Medicago sativa | - |
root nodule | high expression level of SPSA, nodules of plants inoculated with Sinorhizobium meliloti | Medicago sativa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0316 | - |
wild-type leaf isozyme from plants infected with an N-fixation deficient Sinorhizobium meliloti strain | Medicago sativa |
0.0322 | - |
wild-type leaf isozyme from plants infected with a wild-type Sinorhizobium meliloti strain | Medicago sativa |
0.0767 | - |
wild-type nodule isozyme from plants infected with an N-fixation deficient Sinorhizobium meliloti strain | Medicago sativa |
0.0861 | - |
wild-type nodule isozyme from plants infected with a wild-type Sinorhizobium meliloti strain | Medicago sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + D-fructose 6-phosphate | isozyme show differences in allosteric regulation | Medicago sativa | UDP + sucrose 6F-phosphate | - |
? | |
UDP-glucose + D-fructose 6-phosphate | optimal substrate concentrations are 10 mM for D-fructose 6-phosphate and 12 mM for UDP-glucose | Medicago sativa | UDP + sucrose 6F-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | three dfferent isoforms of SPS belonging to two dfferent SPS gene families, SPSA and SPSB in Medicago sativa | Medicago sativa |
SPS | - |
Medicago sativa |
sucrose phosphate synthase | - |
Medicago sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Medicago sativa |
General Information | Comment | Organism |
---|---|---|
metabolism | SPS catalyzes the first step in the synthesis of sucrose in photosynthetic tissue | Medicago sativa |