Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.14 extracted from

  • Pagnussat, G.C.; Curatti, L.; Salerno, G.L.
    Rice sucrose-phosphate synthase: identification of an isoform specific for heterotrophic tissues with distinct metabolite regulation from the mature leaf enzyme (2000), Physiol. Plant., 108, 337-344.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
glucose-6-phosphate SPS-I and SPS-II Oryza sativa

General Stability

General Stability Organism
SPS-I loses more activity during purification than SPS-II Oryza sativa

Inhibitors

Inhibitors Comment Organism Structure
phosphate SPS-I shows high sensitivity towards phosphate, whereas SPS-II is only slightly sensitive Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5
-
D-fructose 6-phosphate SPS-I Oryza sativa
3.6
-
UDP-glucose SPS-I Oryza sativa
4.1
-
D-fructose 6-phosphate SPS-II Oryza sativa
4.2
-
UDP-glucose SPS-II Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
116000
-
SDS-PAGE, minor 88000 Da band detected, that is suggested to be a degradation product Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + D-fructose 6-phosphate Oryza sativa catalyzes the penultimate step of sucrose synthesis UDP + sucrose 6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, two forms of the enzyme identified, SPS-I loses more activity during purification than SPS-II Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Oryza sativa
-
seed germinated Oryza sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + D-fructose 6-phosphate
-
Oryza sativa UDP + sucrose 6-phosphate
-
?
UDP-alpha-D-glucose + D-fructose 6-phosphate catalyzes the penultimate step of sucrose synthesis Oryza sativa UDP + sucrose 6-phosphate
-
?

Synonyms

Synonyms Comment Organism
More two forms of enzyme identified, that differ in regulatory properties Oryza sativa