Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glucose-6-phosphate | only SPS-II is activated, SPS-I not affected | Oryza sativa | |
Mg2+ | stimulates enzyme activity | Oryza sativa |
General Stability | Organism |
---|---|
SPS-I loses more activity during purification than SPS-II | Oryza sativa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphate | only SPS-II is inhibited, SPS-I not affected | Oryza sativa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | stimulates enzyme activity | Oryza sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
116000 | - |
SDS-PAGE, minor 88000 Da band detected, different molecular weight for the two forms of enzyme postulated | Oryza sativa |
420000 | - |
gel filtration | Oryza sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + D-fructose 6-phosphate | Oryza sativa | catalyzes the penultimate step of sucrose synthesis | UDP + sucrose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
rice | - |
Purification (Comment) | Organism |
---|---|
partial, two forms of the enzyme identified, SPS-I loses more activity during purification than SPS-II | Oryza sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Oryza sativa | - |
additional information | no activity in roots | Oryza sativa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.9 | - |
purified enzyme | Oryza sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + D-fructose 6-phosphate | - |
Oryza sativa | UDP + sucrose 6-phosphate | - |
? | |
UDP-alpha-D-glucose + D-fructose 6-phosphate | catalyzes the penultimate step of sucrose synthesis | Oryza sativa | UDP + sucrose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 116000, gel filtration, SDS-PAGE | Oryza sativa |
Synonyms | Comment | Organism |
---|---|---|
More | two forms of enzyme identified, that differ in regulatory properties | Oryza sativa |
More | SPS-I not affected by phosphate or glucose-6-phosphate | Oryza sativa |