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Literature summary for 2.4.1.14 extracted from

  • Salerno, G.L.; Pagnussat, G.C.; Pontis, H.G.
    Studies on sucrose-phosphate synthase from rice leaves (1998), Cell. Mol. Biol., 44, 407-416.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
glucose-6-phosphate only SPS-II is activated, SPS-I not affected Oryza sativa
Mg2+ stimulates enzyme activity Oryza sativa

General Stability

General Stability Organism
SPS-I loses more activity during purification than SPS-II Oryza sativa

Inhibitors

Inhibitors Comment Organism Structure
phosphate only SPS-II is inhibited, SPS-I not affected Oryza sativa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ stimulates enzyme activity Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
116000
-
SDS-PAGE, minor 88000 Da band detected, different molecular weight for the two forms of enzyme postulated Oryza sativa
420000
-
gel filtration Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + D-fructose 6-phosphate Oryza sativa catalyzes the penultimate step of sucrose synthesis UDP + sucrose 6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
rice
-

Purification (Commentary)

Purification (Comment) Organism
partial, two forms of the enzyme identified, SPS-I loses more activity during purification than SPS-II Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Oryza sativa
-
additional information no activity in roots Oryza sativa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.9
-
purified enzyme Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + D-fructose 6-phosphate
-
Oryza sativa UDP + sucrose 6-phosphate
-
?
UDP-alpha-D-glucose + D-fructose 6-phosphate catalyzes the penultimate step of sucrose synthesis Oryza sativa UDP + sucrose 6-phosphate
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 116000, gel filtration, SDS-PAGE Oryza sativa

Synonyms

Synonyms Comment Organism
More two forms of enzyme identified, that differ in regulatory properties Oryza sativa
More SPS-I not affected by phosphate or glucose-6-phosphate Oryza sativa