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Literature summary for 2.4.1.14 extracted from
- Purification and properties of sucrose-phosphate synthase from seeds of Pisum sativum (1990), Phytochemistry, 29, 1057-1063.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose-1,6-diphosphate | - |
Pisum sativum |  |
| glucose-6-phosphate | - |
Pisum sativum | |
| Mg2+ | stimulates enzyme activity | Pisum sativum | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | - |
Pisum sativum | |
| UDP | - |
Pisum sativum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
D-fructose 6-phosphate | in presence of 5 mM glucose 6-phosphate | Pisum sativum | |
| 2.4 | - |
UDP-glucose | - |
Pisum sativum | |
| 3 | - |
D-fructose 6-phosphate | in presence of 0.5 mM glucose 6-phosphate | Pisum sativum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | stimulates enzyme activity | Pisum sativum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 456000 | - |
gel filtration | Pisum sativum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + D-fructose 6-phosphate | Pisum sativum | catalyzes the penultimate step of sucrose synthesis | UDP + sucrose 6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | germinated | Pisum sativum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.22 | - |
purified enzyme | Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + D-fructose 6-phosphate | - |
Pisum sativum | UDP + sucrose 6-phosphate | - |
? | |
| UDP-alpha-D-glucose + D-fructose 6-phosphate | catalyzes the penultimate step of sucrose synthesis | Pisum sativum | UDP + sucrose 6-phosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Pisum sativum |
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