Any feedback?
Literature summary for 2.4.1.14 extracted from
- Kinetic characterization of spinach leaf sucrose phosphate (1982), Plant Physiol., 69, 1027-1030.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 1,5-Anhydroglucitol 6-phosphate | strong stimulation | Spinacia oleracea |  |
General Stability
| General Stability | Organism |
|---|---|
| 2-mercaptoethanol and phenol absorbing agents stabilize activity | Spinacia oleracea |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | competitive with UDP-glucose | Spinacia oleracea | |
| sucrose-6-phosphate | - |
Spinacia oleracea | |
| Tris-HCl buffer | slight inhibition | Spinacia oleracea | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.3 | - |
UDP-glucose | - |
Spinacia oleracea | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | no effect | Spinacia oleracea | |
| Mg2+ | no effect | Spinacia oleracea | |
| Mn2+ | no effect | Spinacia oleracea | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + D-fructose 6-phosphate | Spinacia oleracea | catalyzes the penultimate step of sucrose synthesis | UDP + sucrose 6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| in presence of 2-mercaptoethanol and a phenol absorbing agent under N2 | Spinacia oleracea |
| partial | Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, 4 weeks, 50% loss of activity | Spinacia oleracea |
| liquid nitrogen, stable for at least 11 months | Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + D-fructose 6-phosphate | - |
Spinacia oleracea | UDP + sucrose 6-phosphate | - |
? | |
| UDP-alpha-D-glucose + D-fructose 6-phosphate | catalyzes the penultimate step of sucrose synthesis | Spinacia oleracea | UDP + sucrose 6-phosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | broad optimum | Spinacia oleracea |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
sucrose-6-phosphate | - |
Spinacia oleracea | |
| 1.75 | - |
phosphate | - |
Spinacia oleracea | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
