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Literature summary for 2.4.1.139 extracted from
- Characterization of maltose biosynthesis from alpha-D-gluccose-1-phosphate in Spinacia oleracea L. (1982), Planta, 154, 87-93.
General Stability
| General Stability | Organism |
|---|---|
| during purification addition of 15 mM alpha-D-glucose 1-phosphate to the isolation buffer is necessary for stabilization | Spinacia oleracea |
| purification has to be carried out at 4°C | Spinacia oleracea |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADPglucose | strong | Spinacia oleracea |  |
| ATP | - |
Spinacia oleracea | |
| beta-D-glucose 1-phosphate | - |
Spinacia oleracea | |
| D-glucono-1,5-lactone | competitive | Spinacia oleracea | |
| GDPglucose | strong | Spinacia oleracea | |
| phosphate | - |
Spinacia oleracea | |
| UDPglucose | strong | Spinacia oleracea | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.5 | - |
alpha-D-glucose 1-phosphate | - |
Spinacia oleracea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 95000 | - |
gel filtration | Spinacia oleracea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-glucose 1-phosphate + alpha-D-glucose 1-phosphate | Spinacia oleracea | - |
maltose + phosphate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
spinach | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | - |
Spinacia oleracea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-glucose 1-phosphate + alpha-D-glucose 1-phosphate | - |
Spinacia oleracea | maltose + phosphate + phosphate | - |
? | |
| additional information | exchange of phosphate group of alpha-D-glucose 1-phosphate with phosphate is possible, neither maltose 1-phosphate nor free phosphate can be detected as an intermediate | Spinacia oleracea | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Spinacia oleracea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | 7.5 | 30% of maximal activity at pH 6.2, 45% of maximal activity at pH 7.5 | Spinacia oleracea |
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