Cloned (Comment) | Organism |
---|---|
gene UGT84A25a, seven full-length candidate cDNAs are isolated from Eucalyptus camaldulensis by homology-based cloning and expressed in Escherichia coli as recombinant N-terminal His-tagged proteins, DNA and amino acid sequence determination and analysis, phylogenetic analysis. Reverse transcription quantitative real-time PCR enzyme expression analysis | Eucalyptus camaldulensis |
gene UGT84A25b, seven full-length candidate cDNAs are isolated from Eucalyptus camaldulensis by homology-based cloning and expressed in Escherichia coli as recombinant N-terminal His-tagged proteins, DNA and amino acid sequence determination and analysis, phylogenetic analysis. Reverse transcription quantitative real-time PCR enzyme expression analysis | Eucalyptus camaldulensis |
gene UGT84A26a, seven full-length candidate cDNAs are isolated from Eucalyptus camaldulensis by homology-based cloning and expressed in Escherichia coli as recombinant N-terminal His-tagged proteins, DNA and amino acid sequence determination and analysis, phylogenetic analysis. Reverse transcription quantitative real-time PCR enzyme expression analysis | Eucalyptus camaldulensis |
gene UGT84A26b, seven full-length candidate cDNAs are isolated from Eucalyptus camaldulensis by homology-based cloning and expressed in Escherichia coli as recombinant N-terminal His-tagged proteins, DNA and amino acid sequence determination and analysis, phylogenetic analysis. Reverse transcription quantitative real-time PCR enzyme expression analysis | Eucalyptus camaldulensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.103 | - |
vanillate | pH 6.0, 30°C, recombinant enzyme | Eucalyptus camaldulensis | |
0.111 | - |
vanillate | pH 6.0, 30°C, recombinant enzyme | Eucalyptus camaldulensis | |
0.149 | - |
protocatechuate | pH 6.0, 30°C, recombinant enzyme | Eucalyptus camaldulensis | |
0.168 | - |
gallate | pH 6.0, 30°C, recombinant enzyme | Eucalyptus camaldulensis | |
0.17 | - |
protocatechuate | pH 6.0, 30°C, recombinant enzyme | Eucalyptus camaldulensis | |
0.19 | - |
gallate | pH 6.0, 30°C, recombinant enzyme | Eucalyptus camaldulensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + gallate | Eucalyptus camaldulensis | - |
UDP + 1-galloyl-beta-D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Eucalyptus camaldulensis | A0A2Z5V6P3 | - |
- |
Eucalyptus camaldulensis | A0A2Z5V6P8 | - |
- |
Eucalyptus camaldulensis | A0A2Z5V6P9 | - |
- |
Eucalyptus camaldulensis | A0A2Z5VC92 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Eucalyptus camaldulensis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Eucalyptus camaldulensis | - |
root | - |
Eucalyptus camaldulensis | - |
stem | - |
Eucalyptus camaldulensis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the UGT84A proteins catalyze esterification of UDP-glucose and gallic acid to form 1-O-galloyl-beta-D-glucose. UGT84A25a and -26a also form 1-O-glucose esters of other structurally related hydroxybenzoic and hydroxycinnamic acids with a preference for hydroxybenzoic acids, substrate specificity, overview. UGT84A25b and UGT84A26b are predicted to have similar substrate specificity as UGT84A25a and UGT84A26a. UGT84A25a can be classified as 1-O-ester-forming glucosyltransferases with a preference for hydroxybenzoic acids as glucose acceptor | Eucalyptus camaldulensis | ? | - |
- |
|
additional information | the UGT84A proteins catalyze esterification of UDP-glucose and gallic acid to form 1-O-galloyl-beta-D-glucose. UGT84A25a and -26a also form 1-O-glucose esters of other structurally related hydroxybenzoic and hydroxycinnamic acids with a preference for hydroxybenzoic acids, substrate specificity, overview. UGT84A25b and UGT84A26b are predicted to have similar substrate specificity as UGT84A25a and UGT84A26a. UGT84A26a can be classified as 1-O-ester-forming glucosyltransferases with a preference for hydroxybenzoic acids as glucose acceptor | Eucalyptus camaldulensis | ? | - |
- |
|
additional information | the UGT84A proteins catalyze esterification of UDP-glucose and gallic acid to form 1-O-galloyl-beta-D-glucose. UGT84A25a and -26a also form 1-O-glucose esters of other structurally related hydroxybenzoic and hydroxycinnamic acids with a preference for hydroxybenzoic acids. UGT84A25b and UGT84A26b are predicted to have similar substrate specificity as UGT84A25a and UGT84A26a | Eucalyptus camaldulensis | ? | - |
- |
|
UDP-glucose + caffeate | - |
Eucalyptus camaldulensis | UDP + 1-caffeoyl-beta-D-glucose | - |
? | |
UDP-glucose + ferulate | - |
Eucalyptus camaldulensis | UDP + 1-feruloyl-beta-D-glucose | - |
? | |
UDP-glucose + gallate | - |
Eucalyptus camaldulensis | UDP + 1-galloyl-beta-D-glucose | - |
? | |
UDP-glucose + protocatechuate | - |
Eucalyptus camaldulensis | UDP + 1-protocatechuoyl-beta-D-glucose | - |
? | |
UDP-glucose + sinapate | - |
Eucalyptus camaldulensis | UDP + 1-sinapoyl-beta-D-glucose | - |
? | |
UDP-glucose + vanillate | - |
Eucalyptus camaldulensis | UDP + 1-vanilloyl-beta-D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
1-O-galloyl-beta-D-glucose-forming UGT | - |
Eucalyptus camaldulensis |
EcUGT1a | - |
Eucalyptus camaldulensis |
EcUGT1b | - |
Eucalyptus camaldulensis |
EcUGT2a | - |
Eucalyptus camaldulensis |
EcUGT2b | - |
Eucalyptus camaldulensis |
UDP-glucose:gallate glucosyltransferase | - |
Eucalyptus camaldulensis |
UGT84A25a | - |
Eucalyptus camaldulensis |
UGT84A25b | - |
Eucalyptus camaldulensis |
UGT84A26a | - |
Eucalyptus camaldulensis |
UGT84A26b | - |
Eucalyptus camaldulensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Eucalyptus camaldulensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.1 | - |
protocatechuate | pH 6.0, 30°C, recombinant enzyme | Eucalyptus camaldulensis | |
10.4 | - |
vanillate | pH 6.0, 30°C, recombinant enzyme | Eucalyptus camaldulensis | |
10.7 | - |
gallate | pH 6.0, 30°C, recombinant enzyme | Eucalyptus camaldulensis | |
12.6 | - |
vanillate | pH 6.0, 30°C, recombinant enzyme | Eucalyptus camaldulensis | |
12.7 | - |
protocatechuate | pH 6.0, 30°C, recombinant enzyme | Eucalyptus camaldulensis | |
13.5 | - |
gallate | pH 6.0, 30°C, recombinant enzyme | Eucalyptus camaldulensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Eucalyptus camaldulensis |
Organism | Comment | Expression |
---|---|---|
Eucalyptus camaldulensis | gene expression of UGT84A25a/b and UGT84A26a/b is not induced by either short-term or long-term Al3+ treatment of roots | additional information |
General Information | Comment | Organism |
---|---|---|
evolution | phylogenetic analysis classifies four of seven isolated genes as UDP glycosyltransferase (UGT) 84A subfamily proteins (UGT84A25a, -b, UGT84A26a, -b). In vitro enzyme assays show that the UGT84A proteins catalyze esterification of UDP-glucose and gallic acid to form 1-O-galloyl-beta-D-glucose. UGT84A25a and -26a also form 1-O-glucose esters of other structurally related hydroxybenzoic and hydroxycinnamic acids with a preference for hydroxybenzoic acids | Eucalyptus camaldulensis |
metabolism | the UGT84A subfamily enzymes of Eucalyptus camaldulensis are responsible for constitutive production of 1-O-galloyl-beta-D-glucose, which is the first step of hydrolyzable tannin biosynthesis | Eucalyptus camaldulensis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
56.32 | - |
gallate | pH 6.0, 30°C, recombinant enzyme | Eucalyptus camaldulensis | |
67.79 | - |
protocatechuate | pH 6.0, 30°C, recombinant enzyme | Eucalyptus camaldulensis | |
74.71 | - |
protocatechuate | pH 6.0, 30°C, recombinant enzyme | Eucalyptus camaldulensis | |
80.34 | - |
gallate | pH 6.0, 30°C, recombinant enzyme | Eucalyptus camaldulensis | |
93.69 | - |
vanillate | pH 6.0, 30°C, recombinant enzyme | Eucalyptus camaldulensis | |
122.33 | - |
vanillate | pH 6.0, 30°C, recombinant enzyme | Eucalyptus camaldulensis |