BRENDA - Enzyme Database
show all sequences of 2.4.1.136

Partial purification and properties of UDP-glucose: vanillate 1-O-glucosyl transferase from oak leaves

Gross, G.; Phytochemistry 22, 2179-2182 (1983)
No PubMed abstract available

Data extracted from this reference:

General Stability
General Stability
Organism
2-mercaptoethanol in extraction buffers stabilizes
Quercus rubra
diluted enzyme is unstable
Quercus rubra
Inhibitors
Inhibitors
Commentary
Organism
Structure
dithiothreitol
4 mM, slight
Quercus rubra
UDP
strong
Quercus rubra
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.57
-
vanillate
-
Quercus rubra
0.72
-
veratrate
-
Quercus rubra
1.11
-
gallate
-
Quercus rubra
3.45
-
p-hydroxybenzoate
-
Quercus rubra
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
68000
-
gel filtration
Quercus rubra
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
UDPglucose + gallate
Quercus rubra
physiological role probably is the formation of beta-glucogallin, the putative first intermediate in the biosynthesis of gallotannins
UDP + 1-O-galloyl-beta-D-glucose
i.e. beta-glucogallin
Quercus rubra
r
Organism
Organism
UniProt
Commentary
Textmining
Quercus rubra
-
-
-
Oxidation Stability
Oxidation Stability
Organism
O2-sensitive, mercaptoethanol stabilizes
Quercus rubra
Purification (Commentary)
Purification (Commentary)
Organism
-
Quercus rubra
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Quercus rubra
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.0117
-
reaction with gallate
Quercus rubra
Storage Stability
Storage Stability
Organism
0-4C, 1-2 weeks
Quercus rubra
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
UDP-alpha-D-glucose + 4-hydroxybenzoate
48% of activity compared to vanillate
488579
Quercus rubra
UDP + 1-O-(4-hydroxybenzoyl)-beta-D-glucopyranose
-
-
-
?
UDP-alpha-D-glucose + cinnamate
9% of activity compared to vanillate
488579
Quercus rubra
UDP + 1-O-cinnamoyl-beta-D-glucose
-
-
-
?
UDP-glucose + p-coumarate
32% of activity compared to vanillate
488579
Quercus rubra
UDP + 1-O-p-coumaroyl-beta-D-glucose
-
-
-
?
UDPglucose + 3,4,5-trimethoxybenzoate
7% of activity compared to vanillate
488579
Quercus rubra
UDP + 1-O-3,4,5-trimethoxybenzoyl-beta-D-glucose
-
-
-
?
UDPglucose + anisate
38% of activity compared to vanillate
488579
Quercus rubra
UDP + 1-O-anisoyl-beta-D-glucose
-
-
-
?
UDPglucose + benzoate
16% of activity compared to vanillate
488579
Quercus rubra
UDP + benzoyl-beta-D-glucose
-
-
-
?
UDPglucose + ferulate
35% of activity compared to vanillate
488579
Quercus rubra
UDP + 1-O-feruloyl-beta-D-glucose
-
-
-
?
UDPglucose + gallate
52% of activity compared to vanillate
488579
Quercus rubra
UDP + 1-O-galloyl-beta-D-glucose
i.e. beta-glucogallin
488579
Quercus rubra
r
UDPglucose + gallate
physiological role probably is the formation of beta-glucogallin, the putative first intermediate in the biosynthesis of gallotannins
488579
Quercus rubra
UDP + 1-O-galloyl-beta-D-glucose
i.e. beta-glucogallin
488579
Quercus rubra
r
UDPglucose + m-coumarate
14% of activity compared to vanillate
488579
Quercus rubra
UDP + 1-O-m-coumaroyl-beta-D-glucose
-
-
-
?
UDPglucose + protocatechuate
22% of activity compared to vanillate
488579
Quercus rubra
UDP + protocatechuyl-beta-D-glucose
-
-
-
?
UDPglucose + sinapate
13% of activity compared to vanillate
488579
Quercus rubra
UDP + 1-O-sinapoyl-beta-D-glucose
-
-
-
?
UDPglucose + vanillate
best substrate
488579
Quercus rubra
UDP + vanilloyl-beta-D-glucose
-
-
-
?
UDPglucose + veratrate
71% of activity compared to vanillate
488579
Quercus rubra
UDP + 1-O-veratroyl-beta-D-glucose
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
42
-
-
Quercus rubra
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
7
-
Quercus rubra
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4.7
9
50% of maximal activity at pH 4.7 and 9.0
Quercus rubra
General Stability (protein specific)
General Stability
Organism
2-mercaptoethanol in extraction buffers stabilizes
Quercus rubra
diluted enzyme is unstable
Quercus rubra
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
dithiothreitol
4 mM, slight
Quercus rubra
UDP
strong
Quercus rubra
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.57
-
vanillate
-
Quercus rubra
0.72
-
veratrate
-
Quercus rubra
1.11
-
gallate
-
Quercus rubra
3.45
-
p-hydroxybenzoate
-
Quercus rubra
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
68000
-
gel filtration
Quercus rubra
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
UDPglucose + gallate
Quercus rubra
physiological role probably is the formation of beta-glucogallin, the putative first intermediate in the biosynthesis of gallotannins
UDP + 1-O-galloyl-beta-D-glucose
i.e. beta-glucogallin
Quercus rubra
r
Oxidation Stability (protein specific)
Oxidation Stability
Organism
O2-sensitive, mercaptoethanol stabilizes
Quercus rubra
Purification (Commentary) (protein specific)
Commentary
Organism
-
Quercus rubra
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Quercus rubra
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.0117
-
reaction with gallate
Quercus rubra
Storage Stability (protein specific)
Storage Stability
Organism
0-4C, 1-2 weeks
Quercus rubra
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
UDP-alpha-D-glucose + 4-hydroxybenzoate
48% of activity compared to vanillate
488579
Quercus rubra
UDP + 1-O-(4-hydroxybenzoyl)-beta-D-glucopyranose
-
-
-
?
UDP-alpha-D-glucose + cinnamate
9% of activity compared to vanillate
488579
Quercus rubra
UDP + 1-O-cinnamoyl-beta-D-glucose
-
-
-
?
UDP-glucose + p-coumarate
32% of activity compared to vanillate
488579
Quercus rubra
UDP + 1-O-p-coumaroyl-beta-D-glucose
-
-
-
?
UDPglucose + 3,4,5-trimethoxybenzoate
7% of activity compared to vanillate
488579
Quercus rubra
UDP + 1-O-3,4,5-trimethoxybenzoyl-beta-D-glucose
-
-
-
?
UDPglucose + anisate
38% of activity compared to vanillate
488579
Quercus rubra
UDP + 1-O-anisoyl-beta-D-glucose
-
-
-
?
UDPglucose + benzoate
16% of activity compared to vanillate
488579
Quercus rubra
UDP + benzoyl-beta-D-glucose
-
-
-
?
UDPglucose + ferulate
35% of activity compared to vanillate
488579
Quercus rubra
UDP + 1-O-feruloyl-beta-D-glucose
-
-
-
?
UDPglucose + gallate
52% of activity compared to vanillate
488579
Quercus rubra
UDP + 1-O-galloyl-beta-D-glucose
i.e. beta-glucogallin
488579
Quercus rubra
r
UDPglucose + gallate
physiological role probably is the formation of beta-glucogallin, the putative first intermediate in the biosynthesis of gallotannins
488579
Quercus rubra
UDP + 1-O-galloyl-beta-D-glucose
i.e. beta-glucogallin
488579
Quercus rubra
r
UDPglucose + m-coumarate
14% of activity compared to vanillate
488579
Quercus rubra
UDP + 1-O-m-coumaroyl-beta-D-glucose
-
-
-
?
UDPglucose + protocatechuate
22% of activity compared to vanillate
488579
Quercus rubra
UDP + protocatechuyl-beta-D-glucose
-
-
-
?
UDPglucose + sinapate
13% of activity compared to vanillate
488579
Quercus rubra
UDP + 1-O-sinapoyl-beta-D-glucose
-
-
-
?
UDPglucose + vanillate
best substrate
488579
Quercus rubra
UDP + vanilloyl-beta-D-glucose
-
-
-
?
UDPglucose + veratrate
71% of activity compared to vanillate
488579
Quercus rubra
UDP + 1-O-veratroyl-beta-D-glucose
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
42
-
-
Quercus rubra
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
7
-
Quercus rubra
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4.7
9
50% of maximal activity at pH 4.7 and 9.0
Quercus rubra
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
488579
Gross
-
Partial purification and prope ...
Quercus rubra
Phytochemistry
22
2179-2182
1983
-
-
-
-
-
2
2
4
-
-
1
1
-
1
1
-
1
-
-
1
1
1
14
-
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
4
-
-
1
1
-
1
-
1
-
1
1
1
14
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
488580
Gross
-
Synthesis of beta-glucogallin ...
Quercus rubra
FEBS Lett.
148
67-70
1982
-
-
-
-
-
-
-
2
-
-
-
1
-
1
-
-
1
-
-
1
-
-
2
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
1
-
1
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-