Literature summary for 2.4.1.135 extracted from

Gulberti, S.; Fournel-Gigleux, S.; Mulliert, G.; Aubry, A.; Netter, P.; Magdalou, J.; Ouzzine, M.
The functional glycosyltransferase signature sequence of the human beta 1,3-glucuronosyltransferase is a XDD motif (2003), J. Biol. Chem., 278, 32219-32226.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Pichia pastoris	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
D194A	about 85% of wild-type activity	Homo sapiens
D194A/D195A	inactive mutant protein	Homo sapiens
D194A/D196A	inactive mutant protein	Homo sapiens
D194E	about 85% of wild-type activity	Homo sapiens
D195A	about 25% of wild-type activity	Homo sapiens
D195A/D196A	inactive mutant protein	Homo sapiens
D195E	about 30% of wild-type activity	Homo sapiens
D196A	about 30% of wild-type activity	Homo sapiens
D196E	about 25% of wild-type activity	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	stimulation at 10 mM is 25% of the stimulation with Mn2+. No further activation when the concentration is increased up to 50 mM	Homo sapiens
Mg2+	stimulation at 10 mM is 50% of the stimulation with Mn2+. No further activation when the concentration is increased up to 50 mM	Homo sapiens
Mn2+	0.1-10 mM strongly inactivates. No further activation when the concentration is increased up to 50 mM. Not only essential for GlcAT-I activity but also required for cosubstrate binding. The Asp194-Asp195-Asp196 motif is a major element of the UDP/Mn2+ binding site	Homo sapiens
Zn2+	stimulation at 10 mM is 50% of the stimulation with Mn2+. No further activation when the concentration is increased up to 50 mM	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q9P2W7	-	-

Synonyms

Synonyms	Comment	Organism
GlcAT-I	-	Homo sapiens

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Release 2023.1 (January 2023)