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Literature summary for 2.4.1.135 extracted from

  • Tone, Y.; Kitagawa, H.; Imiya, K.; Oka, S.; Kawasaki, T.; Sugahara, K.
    Characterization of recombinant human glucuronyltransferase I involved in the biosynthesis of the glycosaminoglycan-protein linkage region of proteoglycans (1999), FEBS Lett., 459, 415-420.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expression of full-length GlcAT-I in COS-1 cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0293
-
 UDPglucuronate
-
 Homo sapiens
0.0804
-
 Galbeta(1-3)Galbeta(1-4)Xyl
-
 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ divalent cations essential, 2.7% as effective as Mn2+ Homo sapiens
Mg2+ divalent cations essential, 5.5% as effective as Mn2+ Homo sapiens
Mn2+ divalent cations essential, Mn2+ exhibits highest activity, optimal concentration: 2 mM Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the enzyme is involved in the biosynthesis of the glycosaminoglycan-protein linkage region of proteoglycans ?
-
 ?
UDP-glucuronate + Galbeta(1-3)Galbeta(1-4)Xyl Homo sapiens high expression of the GlcAT-I gene renders the cells capable of synthesizing the HNK-1 epitope UDP + GlcAbeta(1-3)-Galbeta(1-3)Galbeta(1-4)Xyl
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information negligible activity with Galbeta(1-3)galbeta1-O-benzyl, galbeta(1-4)GlcNAc and galbeta(1-4)Glc Homo sapiens ?
-
 ?
additional information the enzyme is involved in the biosynthesis of the glycosaminoglycan-protein linkage region of proteoglycans Homo sapiens ?
-
 ?
UDP-glucuronate + Galbeta(1-3)Galbeta(1-4)Xyl
-
 Homo sapiens UDP + GlcAbeta(1-3)-Galbeta(1-3)Galbeta(1-4)Xyl
-
 ?
UDP-glucuronate + Galbeta(1-3)Galbeta(1-4)Xyl high expression of the GlcAT-I gene renders the cells capable of synthesizing the HNK-1 epitope Homo sapiens UDP + GlcAbeta(1-3)-Galbeta(1-3)Galbeta(1-4)Xyl
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Homo sapiens