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Literature summary for 2.4.1.134 extracted from

  • Robinson, J.A.; Robinson, H.C.
    Initiation of chondroitin sulphate synthesis by beta-D-galactosides. Substrates for galactosyltransferase II (1985), Biochem. J., 227, 805-814.
    View publication on PubMedView publication on EuropePMC
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.05
-
 UDPgalactose pH 6, 37°C, + pyridine 3-O-beta-galactoside Gallus gallus
100
-
 pyridine 3-O-beta-galactoside pH 6, 37°C, value above Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cartilage epiphyses from embryonic femurs and tibiae Gallus gallus
-

Storage Stability

Storage Stability Organism
-20°C, microsomal enzyme preparation, at least 12 months Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-galactose + 4-beta-D-galactosyl-O-beta-D-xylose
-
 Gallus gallus UDP + 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylose
-
 ?
UDP-galactose + beta-galactosides e.g. phenyl or pyridine 3-O-beta-galactosides, poor substrate: pyridine 2-S-beta-thiogalactoside, no substrate: D-galactose Gallus gallus ?
-
 ?
UDP-galactose + galactosyl-xylosylserine
-
 Gallus gallus UDP + 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylserine
-
 ?
UDP-galactose + pyridine 3-O-beta-D-galactoside
-
 Gallus gallus UDP + ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Gallus gallus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 60 min, about 40% loss of activity, beta-galactosides Gallus gallus
60
-
 t1/2: 7 min, beta-galactosides Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 broad, beta-galactosides Gallus gallus