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Literature summary for 2.4.1.133 extracted from

  • Song, H.B.; He, M.; Cai, Z.P.; Huang, K.; Flitsch, S.L.; Liu, L.; Voglmeir, J.
    UDP-glucose 4-epimerase and ?-1,4-galactosyltransferase from the oyster Magallana gigas as valuable biocatalysts for the production of galactosylated products (2018), Int. J. Mol. Sci., 19, 1600 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.133

Cloned(Commentary)

Cloned (Comment) Organism
gene CGI_10013446, transcriptome screening and DNA and amino acid sequence determination and analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Crassostrea gigas

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-galactose + O-beta-D-xylosyl-[protein] Crassostrea gigas
-
 UDP + 4-beta-D-galactosyl-O-beta-D-xylosyl-[protein]
-
 ?

Organism

Organism UniProt Comment Textmining
Crassostrea gigas K1QT40
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Crassostrea gigas

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzymatic galactosylation reaction using epimerase UDP-glucose 4-epimerase MgUGE (EC 5.1.3.2) and beta-1,4-galactosyltransferase MgGalT7 with 4-nitrophenol xylose and UDP-glucose as substrates, mass spectrometric analyses of the acceptor substrate and the galactosylated reaction product, coupled assay method, evaluation, overview Crassostrea gigas ?
-
-
UDP-alpha-D-galactose + O-beta-D-xylosyl-[protein]
-
 Crassostrea gigas UDP + 4-beta-D-galactosyl-O-beta-D-xylosyl-[protein]
-
 ?
UDP-galactose + 4-nitrophenyl-alpha-D-xyloside
-
 Crassostrea gigas UDP + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 37800, recombinant His6-tagged enzyme, SDS-PAGE Crassostrea gigas

Synonyms

Synonyms Comment Organism
beta-1,4-galactosyltransferase
-
 Crassostrea gigas
beta-1,4-galactosyltransferase 7 UniProt Crassostrea gigas
MgGalT7
-
 Crassostrea gigas
pNP-xylose-beta-1,4-galactosyltransferase
-
 Crassostrea gigas

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Crassostrea gigas

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Crassostrea gigas