Literature summary for 2.4.1.133 extracted from

Saliba, M.; Ramalanjaona, N.; Gulberti, S.; Bertin-Jung, I.; Thomas, A.; Dahbi, S.; Lopin-Bon, C.; Jacquinet, J.C.; Breton, C.; Ouzzine, M.; Fournel-Gigleux, S.
Probing the acceptor active site organization of the human recombinant beta1,4-galactosyltransferase 7 and design of xyloside-based inhibitors (2015), J. Biol. Chem., 290, 7658-7670.

Search for more literature for 2.4.1.133

Application

Application	Comment	Organism
drug development	the enzyme is a target for the development of enzyme inhibitors as regulators of glycosaminoglycan synthesis in therapeutics	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Homo sapiens
gene B4GALT7, recombinant expression of GST-tagged wild-type and mutant enzymes in CHOpgsB-618 cells, recombinant expression of the soluble GST-tagged mutant enzyme in Escherichia coli strain BL21(DE3)	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
D211N	inactive	Homo sapiens
H195A	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	Homo sapiens
H195A	the mutant shows reduced activity compared to the wild type enzyme	Homo sapiens
H195Q	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	Homo sapiens
H195Q	the mutant shows reduced activity compared to the wild type enzyme	Homo sapiens
H195R	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	Homo sapiens
H195R	the mutant shows reduced activity compared to the wild type enzyme	Homo sapiens
additional information	construction of a soluble enzyme mutant by truncation of the first 60 N-terminal amino acids	Homo sapiens
R226A	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	Homo sapiens
R226A	the mutant shows reduced activity compared to the wild type enzyme	Homo sapiens
R226K	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	Homo sapiens
R226K	the mutant shows reduced activity compared to the wild type enzyme	Homo sapiens
R270A	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	Homo sapiens
R270A	the mutant shows reduced activity compared to the wild type enzyme	Homo sapiens
R270K	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	Homo sapiens
R270K	the mutant shows reduced activity compared to the wild type enzyme	Homo sapiens
Y194A	inactive	Homo sapiens
Y194A	site-directed mutagenesis, inactive mutant	Homo sapiens
Y194F	inactive	Homo sapiens
Y194F	site-directed mutagenesis, inactive mutant	Homo sapiens
Y196A	inactive	Homo sapiens
Y196A	site-directed mutagenesis, inactive mutant	Homo sapiens
Y196F	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	Homo sapiens
Y196F	the mutant shows reduced activity compared to the wild type enzyme	Homo sapiens
Y199A	inactive	Homo sapiens
Y199A	site-directed mutagenesis, inactive mutant	Homo sapiens
Y199F	site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme	Homo sapiens
Y199F	the mutant shows reduced activity compared to the wild type enzyme	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism
4-methylumbelliferyl 4-deoxy-beta-D-fluoroxylose	-	Homo sapiens
4-methylumbelliferyl 4-deoxy-beta-D-xylopyranoside	-	Homo sapiens
4-methylumbelliferyl 4-deoxy-beta-D-xyloside	-	Homo sapiens
4-methylumbelliferyl 4-fluoro-beta-D-xylopyranoside	50% inhibition at 2 mM. The strategic position of Tyr194 forming stacking interactions with the aglycone, and the hydrogen bond between the His195 nitrogen backbone and the carbonyl group of the coumarinyl molecule to develop a tight binder of the enzyme. This leads to the synthesis of 4-deoxy-4-fluoroxylose linked to 4-methylumbelliferone that inhibits enzyme activity in vitro with a Ki 10 times lower than the Km value and efficiently impairs glycosaminoglycan synthesis in a cell assay	Homo sapiens
additional information	not inhibited by naphthyl 4-deoxy-beta-D-xylopyranoside; structure-guided design of enzyme inhibitors, xyloside inhibitors design and in vitro and in cellulo competition assays, overview	Homo sapiens
naphthyl 4-deoxy-beta-D-xylopyranoside	slight inhibition	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
additional information	-	additional information	Michaelis-Menten kinetics	Homo sapiens
0.22	-	UDP-alpha-D-galactose	pH 7.0, 37°C, recombinant wild-type enzyme	Homo sapiens
0.22	-	UDP-alpha-D-galactose	wild type enzyme, at pH 7.0 and 37°C	Homo sapiens
0.27	-	UDP-alpha-D-galactose	mutant enzyme R270A, at pH 7.0 and 37°C	Homo sapiens
0.27	-	UDP-alpha-D-galactose	pH 7.0, 37°C, recombinant mutant R270A	Homo sapiens
0.29	-	UDP-alpha-D-galactose	mutant enzyme R226K, at pH 7.0 and 37°C	Homo sapiens
0.29	-	UDP-alpha-D-galactose	pH 7.0, 37°C, recombinant mutant R226K	Homo sapiens
0.32	-	UDP-alpha-D-galactose	mutant enzyme H195R, at pH 7.0 and 37°C	Homo sapiens
0.32	-	UDP-alpha-D-galactose	mutant enzyme Y199F, at pH 7.0 and 37°C	Homo sapiens
0.32	-	UDP-alpha-D-galactose	pH 7.0, 37°C, recombinant mutant H195R	Homo sapiens
0.32	-	UDP-alpha-D-galactose	pH 7.0, 37°C, recombinant mutant Y199F	Homo sapiens
0.33	-	UDP-alpha-D-galactose	mutant enzyme H195Q, at pH 7.0 and 37°C	Homo sapiens
0.33	-	UDP-alpha-D-galactose	pH 7.0, 37°C, recombinant mutant H195Q	Homo sapiens
0.34	-	UDP-alpha-D-galactose	mutant enzyme R226A, at pH 7.0 and 37°C	Homo sapiens
0.34	-	UDP-alpha-D-galactose	mutant enzyme Y196F, at pH 7.0 and 37°C	Homo sapiens
0.34	-	UDP-alpha-D-galactose	pH 7.0, 37°C, recombinant mutant R226A	Homo sapiens
0.34	-	UDP-alpha-D-galactose	pH 7.0, 37°C, recombinant mutant Y196F	Homo sapiens
0.35	-	4-methylumbelliferyl-beta-D-xylopyranoside	pH 7.0, 37°C, recombinant wild-type enzyme	Homo sapiens
0.35	-	4-methylumbelliferyl alpha-D-xylopyranoside	wild type enzyme, at pH 7.0 and 37°C	Homo sapiens
0.35	-	4-methylumbelliferyl alpha-D-xylopyranoside	mutant enzyme H195R, at pH 7.0 and 37°C	Homo sapiens
0.35	-	4-methylumbelliferyl-beta-D-xylopyranoside	pH 7.0, 37°C, recombinant mutant H195R	Homo sapiens
0.37	-	UDP-alpha-D-galactose	mutant enzyme R270K, at pH 7.0 and 37°C	Homo sapiens
0.37	-	UDP-alpha-D-galactose	pH 7.0, 37°C, recombinant mutant R270K	Homo sapiens
0.4	-	UDP-alpha-D-galactose	mutant enzyme H195A, at pH 7.0 and 37°C	Homo sapiens
0.4	-	UDP-alpha-D-galactose	pH 7.0, 37°C, recombinant mutant H195A	Homo sapiens
0.44	-	4-methylumbelliferyl alpha-D-xylopyranoside	mutant enzyme R226A, at pH 7.0 and 37°C	Homo sapiens
0.44	-	4-methylumbelliferyl-beta-D-xylopyranoside	pH 7.0, 37°C, recombinant mutant R226A	Homo sapiens
0.46	-	4-methylumbelliferyl alpha-D-xylopyranoside	mutant enzyme R226K, at pH 7.0 and 37°C	Homo sapiens
0.46	-	4-methylumbelliferyl-beta-D-xylopyranoside	pH 7.0, 37°C, recombinant mutant R226K	Homo sapiens
0.54	-	4-methylumbelliferyl alpha-D-xylopyranoside	mutant enzyme R270K, at pH 7.0 and 37°C	Homo sapiens
0.54	-	4-methylumbelliferyl-beta-D-xylopyranoside	pH 7.0, 37°C, recombinant mutant R270K	Homo sapiens
0.55	-	4-methylumbelliferyl alpha-D-xylopyranoside	mutant enzyme H195Q, at pH 7.0 and 37°C	Homo sapiens
0.55	-	4-methylumbelliferyl-beta-D-xylopyranoside	pH 7.0, 37°C, recombinant mutant H195Q	Homo sapiens
0.59	-	4-methylumbelliferyl alpha-D-xylopyranoside	mutant enzyme Y199F, at pH 7.0 and 37°C	Homo sapiens
0.59	-	4-methylumbelliferyl-beta-D-xylopyranoside	pH 7.0, 37°C, recombinant mutant Y199F	Homo sapiens
0.6	-	4-methylumbelliferyl alpha-D-xylopyranoside	mutant enzyme R270A, at pH 7.0 and 37°C	Homo sapiens
0.6	-	4-methylumbelliferyl-beta-D-xylopyranoside	pH 7.0, 37°C, recombinant mutant R270A	Homo sapiens
0.64	-	4-methylumbelliferyl alpha-D-xylopyranoside	mutant enzyme H195A, at pH 7.0 and 37°C	Homo sapiens
0.64	-	4-methylumbelliferyl-beta-D-xylopyranoside	pH 7.0, 37°C, recombinant mutant H195A	Homo sapiens
1.06	-	4-methylumbelliferyl alpha-D-xylopyranoside	mutant enzyme Y196F, at pH 7.0 and 37°C	Homo sapiens
1.06	-	4-methylumbelliferyl-beta-D-xylopyranoside	pH 7.0, 37°C, recombinant mutant Y196F	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	required	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
39000	-	x * 39000, SDS-PAGE	Homo sapiens
39000	-	x * 39000, glycosylated recombinant enzyme, SDS-PAGE	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q9UBV7	-	-
Homo sapiens	Q9UBV7	gene B4GALT7	-

Purification (Commentary)

Purification (Comment)	Organism
glutathione Sepharose column chromatography	Homo sapiens
recombinant soluble GST-tagged mutant enzyme from Escherichia coli strain BL21(DE3)	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-alpha-D-galactose + 4-methylumbelliferyl alpha-D-xylopyranoside	-	Homo sapiens	UDP + 4-methylumbelliferyl 4-O-beta-D-galactopyranosyl-beta-D-xylopyranoside	-	?
UDP-alpha-D-galactose + 4-methylumbelliferyl-beta-D-xylopyranoside	the strategic position of Tyr194 forming stacking interactions with the aglycone, and the hydrogen bond between the His195 nitrogen backbone and the carbonyl group of the coumarinyl molecule to develop a tight binder of the enzyme. This leads to the synthesis of 4-deoxy-4-fluoroxylose linked to 4-methylumbelliferone that inhibits enzyme activity in vitro with a Ki 10 times lower than the Km value and efficiently impairs glycosaminoglycan synthesis in a cell assay. Molecular modeling of the enzyme's active site in the presence of UDP-alpha-D-galactose and 4-methylumbelliferyl-beta-D-xylopyranoside using the crystal structure of Drosophila melanogaster enzyme dbeta4GalT7, PDB code 4M4K, overview	Homo sapiens	UDP + ?	-	?

Subunits

Subunits	Comment	Organism
?	x * 39000, SDS-PAGE	Homo sapiens
?	x * 39000, glycosylated recombinant enzyme, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
beta1,4-galactosyltransferase 7	-	Homo sapiens
beta4GalT7	-	Homo sapiens
hbeta4GalT7	-	Homo sapiens
xylosylprotein beta1,4-galactosyltransferase	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
0.5	-	UDP-alpha-D-galactose	mutant enzyme Y196F, at pH 7.0 and 37°C	Homo sapiens
0.5	-	UDP-alpha-D-galactose	pH 7.0, 37°C, recombinant mutant Y196F	Homo sapiens
0.5	-	4-methylumbelliferyl-beta-D-xylopyranoside	pH 7.0, 37°C, recombinant mutant Y196F	Homo sapiens
0.77	-	UDP-alpha-D-galactose	mutant enzyme R270A, at pH 7.0 and 37°C	Homo sapiens
0.77	-	UDP-alpha-D-galactose	pH 7.0, 37°C, recombinant mutant R270A	Homo sapiens
0.77	-	4-methylumbelliferyl-beta-D-xylopyranoside	pH 7.0, 37°C, recombinant mutant R270A	Homo sapiens
0.81	-	UDP-alpha-D-galactose	mutant enzyme R270K, at pH 7.0 and 37°C	Homo sapiens
0.81	-	UDP-alpha-D-galactose	pH 7.0, 37°C, recombinant mutant R270K	Homo sapiens
0.81	-	4-methylumbelliferyl-beta-D-xylopyranoside	pH 7.0, 37°C, recombinant mutant R270K	Homo sapiens
0.89	-	UDP-alpha-D-galactose	mutant enzyme R226A, at pH 7.0 and 37°C	Homo sapiens
0.89	-	UDP-alpha-D-galactose	pH 7.0, 37°C, recombinant mutant R226A	Homo sapiens
0.89	-	4-methylumbelliferyl-beta-D-xylopyranoside	pH 7.0, 37°C, recombinant mutant R226A	Homo sapiens
1.21	-	UDP-alpha-D-galactose	mutant enzyme Y199F, at pH 7.0 and 37°C	Homo sapiens
1.21	-	UDP-alpha-D-galactose	pH 7.0, 37°C, recombinant mutant Y199F	Homo sapiens
1.21	-	4-methylumbelliferyl-beta-D-xylopyranoside	pH 7.0, 37°C, recombinant mutant Y199F	Homo sapiens
1.35	-	UDP-alpha-D-galactose	mutant enzyme R226K, at pH 7.0 and 37°C	Homo sapiens
1.35	-	UDP-alpha-D-galactose	pH 7.0, 37°C, recombinant mutant R226K	Homo sapiens
1.35	-	4-methylumbelliferyl-beta-D-xylopyranoside	pH 7.0, 37°C, recombinant mutant R226K	Homo sapiens
1.49	-	UDP-alpha-D-galactose	mutant enzyme H195R, at pH 7.0 and 37°C	Homo sapiens
1.49	-	UDP-alpha-D-galactose	pH 7.0, 37°C, recombinant mutant H195R	Homo sapiens
1.49	-	4-methylumbelliferyl-beta-D-xylopyranoside	pH 7.0, 37°C, recombinant mutant H195R	Homo sapiens
1.5	-	UDP-alpha-D-galactose	pH 7.0, 37°C, recombinant wild-type enzyme	Homo sapiens
1.5	-	4-methylumbelliferyl-beta-D-xylopyranoside	pH 7.0, 37°C, recombinant wild-type enzyme	Homo sapiens
1.51	-	UDP-alpha-D-galactose	wild type enzyme, at pH 7.0 and 37°C	Homo sapiens
1.62	-	UDP-alpha-D-galactose	mutant enzyme H195Q, at pH 7.0 and 37°C	Homo sapiens
1.62	-	UDP-alpha-D-galactose	pH 7.0, 37°C, recombinant mutant H195Q	Homo sapiens
1.62	-	4-methylumbelliferyl-beta-D-xylopyranoside	pH 7.0, 37°C, recombinant mutant H195Q	Homo sapiens
1.93	-	UDP-alpha-D-galactose	mutant enzyme H195A, at pH 7.0 and 37°C	Homo sapiens
1.93	-	UDP-alpha-D-galactose	pH 7.0, 37°C, recombinant mutant H195A	Homo sapiens
1.93	-	4-methylumbelliferyl-beta-D-xylopyranoside	pH 7.0, 37°C, recombinant mutant H195A	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism
0.03	-	4-methylumbelliferyl 4-deoxy-beta-D-fluoroxylose	at pH 7.0 and 37°C	Homo sapiens
0.03	-	4-methylumbelliferyl 4-fluoro-beta-D-xylopyranoside	pH 7.0, 37°C, recombinant wild-type enzyme	Homo sapiens
0.53	-	4-methylumbelliferyl 4-deoxy-beta-D-xyloside	at pH 7.0 and 37°C	Homo sapiens
0.53	-	4-methylumbelliferyl 4-deoxy-beta-D-xylopyranoside	pH 7.0, 37°C, recombinant wild-type enzyme	Homo sapiens

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor
0.06	-	at pH 7.0 and 37°C	Homo sapiens	4-methylumbelliferyl 4-deoxy-beta-D-fluoroxylose
0.06	-	pH 7.0, 37°C, recombinant wild-type enzyme	Homo sapiens	4-methylumbelliferyl 4-fluoro-beta-D-xylopyranoside
1.28	-	at pH 7.0 and 37°C	Homo sapiens	4-methylumbelliferyl 4-deoxy-beta-D-xyloside
1.28	-	pH 7.0, 37°C, recombinant wild-type enzyme	Homo sapiens	4-methylumbelliferyl 4-deoxy-beta-D-xylopyranoside

General Information

General Information	Comment	Organism
additional information	mapping of the organization of the acceptor binding pockex in complex with 4-methylumbelliferone-xylopyranoside as prototype substrate by combining molecular modeling, in vitro mutagenesis, and kinetic measurements, and in cellulo analysis of glycosaminoglycan anabolism and decorin glycosylation. The organization is governed, on one side, by three tyrosine residues, Tyr194, Tyr196, and Tyr199, which create a hydrophobic environment and provide stacking interactions with both xylopyranoside and aglycone rings. On the opposite side, a hydrogen-bond network is established between the charged amino acids Asp228, Asp229, and Arg226, and the hydroxyl groups of xylose	Homo sapiens
physiological function	human xylosylprotein beta1,4-galactosyltransferase catalyzes the transfer of the first Gal residue of the tetrasaccharide linkage from the activated sugar UDP-galactose onto Xyl residues attached to the proteoglycan core protein. Because all glycosaminoglycans share the same stem core tetrasaccharide, the enzyme is a central enzyme in glycosaminoglycan biosynthesis	Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism
0.47	-	4-methylumbelliferyl alpha-D-xylopyranoside	mutant enzyme Y196F, at pH 7.0 and 37°C	Homo sapiens
0.47	-	4-methylumbelliferyl-beta-D-xylopyranoside	pH 7.0, 37°C, recombinant mutant Y196F	Homo sapiens
1.2	-	4-methylumbelliferyl alpha-D-xylopyranoside	mutant enzyme R270A, at pH 7.0 and 37°C	Homo sapiens
1.2	-	4-methylumbelliferyl-beta-D-xylopyranoside	pH 7.0, 37°C, recombinant mutant R270A	Homo sapiens
1.42	-	4-methylumbelliferyl alpha-D-xylopyranoside	mutant enzyme R270K, at pH 7.0 and 37°C	Homo sapiens
1.42	-	4-methylumbelliferyl-beta-D-xylopyranoside	pH 7.0, 37°C, recombinant mutant R270K	Homo sapiens
1.47	-	UDP-alpha-D-galactose	mutant enzyme Y196F, at pH 7.0 and 37°C	Homo sapiens
1.47	-	UDP-alpha-D-galactose	pH 7.0, 37°C, recombinant mutant Y196F	Homo sapiens
1.87	-	4-methylumbelliferyl alpha-D-xylopyranoside	mutant enzyme R226A, at pH 7.0 and 37°C	Homo sapiens
1.87	-	4-methylumbelliferyl-beta-D-xylopyranoside	pH 7.0, 37°C, recombinant mutant R226A	Homo sapiens
1.88	-	4-methylumbelliferyl alpha-D-xylopyranoside	mutant enzyme Y199F, at pH 7.0 and 37°C	Homo sapiens
1.88	-	4-methylumbelliferyl-beta-D-xylopyranoside	pH 7.0, 37°C, recombinant mutant Y199F	Homo sapiens
2.32	-	UDP-alpha-D-galactose	mutant enzyme R270K, at pH 7.0 and 37°C	Homo sapiens
2.32	-	UDP-alpha-D-galactose	pH 7.0, 37°C, recombinant mutant R270K	Homo sapiens
2.85	-	UDP-alpha-D-galactose	mutant enzyme R226A, at pH 7.0 and 37°C	Homo sapiens
2.85	-	UDP-alpha-D-galactose	pH 7.0, 37°C, recombinant mutant R226A	Homo sapiens
2.92	-	4-methylumbelliferyl alpha-D-xylopyranoside	mutant enzyme R226K, at pH 7.0 and 37°C	Homo sapiens
2.92	-	4-methylumbelliferyl-beta-D-xylopyranoside	pH 7.0, 37°C, recombinant mutant R226K	Homo sapiens
2.95	-	4-methylumbelliferyl alpha-D-xylopyranoside	mutant enzyme H195Q, at pH 7.0 and 37°C	Homo sapiens
2.95	-	4-methylumbelliferyl-beta-D-xylopyranoside	pH 7.0, 37°C, recombinant mutant H195Q	Homo sapiens
3	-	4-methylumbelliferyl alpha-D-xylopyranoside	mutant enzyme H195A, at pH 7.0 and 37°C	Homo sapiens
3	-	4-methylumbelliferyl-beta-D-xylopyranoside	pH 7.0, 37°C, recombinant mutant H195A	Homo sapiens
3.07	-	UDP-alpha-D-galactose	mutant enzyme R270A, at pH 7.0 and 37°C	Homo sapiens
3.07	-	UDP-alpha-D-galactose	pH 7.0, 37°C, recombinant mutant R270A	Homo sapiens
4.03	-	4-methylumbelliferyl alpha-D-xylopyranoside	mutant enzyme H195R, at pH 7.0 and 37°C	Homo sapiens
4.03	-	4-methylumbelliferyl-beta-D-xylopyranoside	pH 7.0, 37°C, recombinant mutant H195R	Homo sapiens
4.05	-	UDP-alpha-D-galactose	mutant enzyme Y199F, at pH 7.0 and 37°C	Homo sapiens
4.05	-	UDP-alpha-D-galactose	pH 7.0, 37°C, recombinant mutant Y199F	Homo sapiens
4.17	-	4-methylumbelliferyl alpha-D-xylopyranoside	wild type enzyme, at pH 7.0 and 37°C	Homo sapiens
4.17	-	4-methylumbelliferyl-beta-D-xylopyranoside	pH 7.0, 37°C, recombinant wild-type enzyme	Homo sapiens
4.7	-	UDP-alpha-D-galactose	mutant enzyme R226K, at pH 7.0 and 37°C	Homo sapiens
4.7	-	UDP-alpha-D-galactose	pH 7.0, 37°C, recombinant mutant R226K	Homo sapiens
4.85	-	UDP-alpha-D-galactose	mutant enzyme H195A, at pH 7.0 and 37°C	Homo sapiens
4.85	-	UDP-alpha-D-galactose	mutant enzyme H195R, at pH 7.0 and 37°C	Homo sapiens
4.85	-	UDP-alpha-D-galactose	pH 7.0, 37°C, recombinant mutant H195A	Homo sapiens
4.92	-	UDP-alpha-D-galactose	pH 7.0, 37°C, recombinant mutant H195Q	Homo sapiens
4.92	-	UDP-alpha-D-galactose	pH 7.0, 37°C, recombinant mutant H195R	Homo sapiens
7.08	-	UDP-alpha-D-galactose	wild type enzyme, at pH 7.0 and 37°C	Homo sapiens
7.08	-	UDP-alpha-D-galactose	pH 7.0, 37°C, recombinant wild-type enzyme	Homo sapiens