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Literature summary for 2.4.1.133 extracted from

  • Talhaoui, I.; Bui, C.; Oriol, R.; Mulliert, G.; Gulberti, S.; Netter, P.; Coughtrie, M.W.; Ouzzine, M.; Fournel-Gigleux, S.
    Identification of key functional residues in the active site of human {beta}1,4-galactosyltransferase 7: a major enzyme in the glycosaminoglycan synthesis pathway (2010), J. Biol. Chem., 285, 37342-37358.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.133

Cloned(Commentary)

Cloned (Comment) Organism
expressed as a GST-fusion protein in Escherichia coli and as Myc-tagged fusion proteins in HeLa cells Homo sapiens
expressed in Escherichia coli BL21(DE3) cells, HeLa cells and CHO pgsB-618 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
D163A inactive Homo sapiens
D165A inactive Homo sapiens
D165E the apparent Km values of the D165E mutant toward UDP-galactose and 4-methylumbelliferyl-O-beta-D-xylopyranoside are about 3.8 and 5.5fold, respectively, higher than the wild type enzyme Homo sapiens
D228A inactive Homo sapiens
D228E the mutant expressed in HeLa cells retains about 40% activity of the wild type enzyme Homo sapiens
D230A the mutant shows increased Km values towards UDP-galactose and 4-methylumbelliferyl-O-beta-D-xylopyranoside compared to the wild type enzyme Homo sapiens
E227D the mutant displays similar apparent Km values toward both donor and acceptor substrates compared with the wild type enzyme Homo sapiens
F221A the mutant shows increased Km values towards UDP-galactose and 4-methylumbelliferyl-O-beta-D-xylopyranoside compared to the wild type enzyme Homo sapiens
F221Y the mutant shows increased Km values towards UDP-galactose and 4-methylumbelliferyl-O-beta-D-xylopyranoside compared to the wild type enzyme Homo sapiens
G223a the mutant is about 40% less active than the wild type enzyme Homo sapiens
G225A the mutant does not display any in vitro activity Homo sapiens
V164A the mutant shows increased Km values towards UDP-galactose and 4-methylumbelliferyl-O-beta-D-xylopyranoside compared to the wild type enzyme Homo sapiens
W222A the mutant shows decreased Km value towards UDP-galactose compared to the wild type enzyme Homo sapiens
W222F the mutation has no significant effect on the Km value toward donor or acceptor substrate Homo sapiens
W224H the mutant is able to sustain decorin glycosaminoglycan chain substitution but not glycosaminoglycan synthesis from exogenously added xyloside Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 UDP-galactose pH 7.0, 37°C, mutant W224H, protein expressed in Escherichia coli Homo sapiens
0.07
-
 UDP-galactose pH 7.0, 37°C, mutant W224H, protein expressed in HeLa cells Homo sapiens
0.07
-
 UDP-galactose mutant enzyme W224H, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
0.1
-
 UDP-galactose pH 7.0, 37°C, mutant E227D, protein expressed in Escherichia coli Homo sapiens
0.16
-
 4-methylumbelliferyl-beta-D-xylopyranoside pH 7.0, 37°C, mutant W222F, protein expressed in HeLa cells Homo sapiens
0.21
-
 UDP-galactose pH 7.0, 37°C, mutant W222A, protein expressed in HeLa cells Homo sapiens
0.21
-
 UDP-galactose mutant enzyme W222A, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
0.28
-
 UDP-galactose pH 7.0, 37°C, wild-type, protein expressed in Escherichia coli Homo sapiens
0.31
-
 UDP-galactose pH 7.0, 37°C, wild-type, protein expressed in HeLa cells Homo sapiens
0.31
-
 UDP-galactose wild type enzyme, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
0.32
-
 4-methylumbelliferyl-beta-D-xylopyranoside pH 7.0, 37°C, wild-type, protein expressed in HeLa cells Homo sapiens
0.32
-
 4-methylumbelliferyl O-beta-D-xylopyranoside wild type enzyme, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
0.33
-
 UDP-galactose pH 7.0, 37°C, mutant F221Y, protein expressed in HeLa cells Homo sapiens
0.33
-
 4-methylumbelliferyl-beta-D-xylopyranoside pH 7.0, 37°C, wild-type, protein expressed in Escherichia coli Homo sapiens
0.33
-
 UDP-galactose mutant enzyme F221Y, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
0.36
-
 UDP-galactose pH 7.0, 37°C, mutant F221A, protein expressed in HeLa cells Homo sapiens
0.36
-
 UDP-galactose mutant enzyme F221A, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
0.38
-
 4-methylumbelliferyl-beta-D-xylopyranoside pH 7.0, 37°C, mutant F221Y, protein expressed in HeLa cells Homo sapiens
0.38
-
 4-methylumbelliferyl O-beta-D-xylopyranoside mutant enzyme F221Y, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
0.43
-
 UDP-galactose pH 7.0, 37°C, mutant D230A, protein expressed in HeLa cells Homo sapiens
0.43
-
 UDP-galactose mutant enzyme D230A, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
0.45
-
 UDP-galactose pH 7.0, 37°C, mutant G223A, protein expressed in HeLa cells Homo sapiens
0.45
-
 UDP-galactose pH 7.0, 37°C, mutant W222F, protein expressed in HeLa cells Homo sapiens
0.45
-
 UDP-galactose mutant enzyme G223A, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
0.45
-
 UDP-galactose mutant enzyme W222F, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
0.47
-
 UDP-galactose pH 7.0, 37°C, mutant D228E, protein expressed in HeLa cells Homo sapiens
0.47
-
 UDP-galactose pH 7.0, 37°C, mutant E227D, protein expressed in HeLa cells Homo sapiens
0.47
-
 UDP-galactose mutant enzyme D228E, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
0.47
-
 UDP-galactose mutant enzyme E227D, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
0.48
-
 UDP-galactose pH 7.0, 37°C, mutant V164A, protein expressed in HeLa cells Homo sapiens
0.48
-
 UDP-galactose mutant enzyme V164A, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
0.52
-
 4-methylumbelliferyl-beta-D-xylopyranoside pH 7.0, 37°C, mutant E227D, protein expressed in HeLa cells Homo sapiens
0.53
-
 4-methylumbelliferyl-beta-D-xylopyranoside pH 7.0, 37°C, mutant V164A, protein expressed in HeLa cells Homo sapiens
0.53
-
 4-methylumbelliferyl O-beta-D-xylopyranoside mutant enzyme V164A, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
0.57
-
 4-methylumbelliferyl-beta-D-xylopyranoside pH 7.0, 37°C, mutant D230A, protein expressed in HeLa cells Homo sapiens
0.73
-
 4-methylumbelliferyl-beta-D-xylopyranoside pH 7.0, 37°C, mutant G223A, protein expressed in HeLa cells Homo sapiens
0.74
-
 4-methylumbelliferyl-beta-D-xylopyranoside pH 7.0, 37°C, mutant E227D, protein expressed in Escherichia coli Homo sapiens
1.17
-
 UDP-galactose pH 7.0, 37°C, mutant D165E, protein expressed in HeLa cells Homo sapiens
1.17
-
 UDP-galactose mutant enzyme D165E, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
1.76
-
 4-methylumbelliferyl-beta-D-xylopyranoside pH 7.0, 37°C, mutant D165E, protein expressed in HeLa cells Homo sapiens
1.76
-
 4-methylumbelliferyl O-beta-D-xylopyranoside mutant enzyme D165E, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens
2.57
-
 4-methylumbelliferyl-beta-D-xylopyranoside pH 7.0, 37°C, mutant D228E, protein expressed in HeLa cells Homo sapiens
4
-
 4-methylumbelliferyl-beta-D-xylopyranoside pH 7.0, 37°C, mutant W224H, value above 4, protein expressed in HeLa cells Homo sapiens
4.22
-
 4-methylumbelliferyl-beta-D-xylopyranoside pH 7.0, 37°C, mutant F221A, protein expressed in HeLa cells Homo sapiens
4.22
-
 4-methylumbelliferyl O-beta-D-xylopyranoside mutant enzyme F221A, in 100 mM sodium cacodylate buffer (pH 7.0) containing 10 mM MnCl2, at 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UBV7
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione-Sepharose column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-galactose + 4-methylumbelliferyl O-beta-D-xylopyranoside
-
 Homo sapiens UDP + 4-methylumbelliferyl 4-O-beta-D-galactopyranosyl-beta-D-xylopyranoside
-
 ?
UDP-galactose + 4-methylumbelliferyl-beta-D-xylopyranoside
-
 Homo sapiens ?
-
 ?
UDP-galactose + 4-nitrophenyl O-beta-D-xylopyranoside
-
 Homo sapiens UDP + 4-nitrophenyl 4-O-beta-D-galactopyranosyl-beta-D-xylopyranoside
-
 ?

Synonyms

Synonyms Comment Organism
beta1,4-galactosyltransferase 7
-
 Homo sapiens
beta4GalT7
-
 Homo sapiens
xylosylprotein beta1,4-galactosyltransferase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 UDP-galactose pH 7.0, 37°C, mutant W224H, protein expressed in Escherichia coli Homo sapiens
0.13
-
 4-methylumbelliferyl-beta-D-xylopyranoside pH 7.0, 37°C, mutant E227D, protein expressed in Escherichia coli Homo sapiens
0.46
-
 UDP-galactose pH 7.0, 37°C, mutant E227D, protein expressed in Escherichia coli Homo sapiens
1.52
-
 UDP-galactose pH 7.0, 37°C, wild-type, protein expressed in Escherichia coli Homo sapiens
1.79
-
 4-methylumbelliferyl-beta-D-xylopyranoside pH 7.0, 37°C, wild-type, protein expressed in Escherichia coli Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.17
-
 4-methylumbelliferyl-beta-D-xylopyranoside pH 7.0, 37°C, mutant E227D, protein expressed in Escherichia coli Homo sapiens
4.6
-
 UDP-galactose pH 7.0, 37°C, mutant E227D, protein expressed in Escherichia coli Homo sapiens
5
-
 UDP-galactose pH 7.0, 37°C, mutant W224H, protein expressed in Escherichia coli Homo sapiens
5.42
-
 4-methylumbelliferyl-beta-D-xylopyranoside pH 7.0, 37°C, wild-type, protein expressed in Escherichia coli Homo sapiens
5.43
-
 UDP-galactose pH 7.0, 37°C, wild-type, protein expressed in Escherichia coli Homo sapiens