Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.133 extracted from

  • Daligault, F.; Rahuel-Clermont, S.; Gulberti, S.; Cung, M.T.; Branlant, G.; Netter, P.; Magdalou, J.; Lattard, V.
    Thermodynamic insights into the structural basis governing the donor substrate recognition by human beta1,4-galactosyltransferase 7 (2009), Biochem. J., 418, 605-614.
    View publication on PubMed
Search for more literature for 2.4.1.133

Cloned(Commentary)

Cloned (Comment) Organism
cloning and expression of human membrane beta1,4-GalT7 in HeLa cells, expression of soluble form as a fusion protein with maltose-binding protein in Escherichia coli Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
CDP
-
 Homo sapiens
TDP
-
 Homo sapiens
UDP
-
 Homo sapiens
UDP-mannose
-
 Homo sapiens
UDP-N-acetylgalactosamine
-
 Homo sapiens
UTP
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 UDP-N-acetyl glucosamine KM value is above 10 in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 Homo sapiens
additional information
-
 UDP-glucuronic acid KM value is above 4 in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 Homo sapiens
additional information
-
 UDP-N-acetyl galactosamine KM value is not detected in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 Homo sapiens
additional information
-
 UDP-mannose KM value is not detected, in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 Homo sapiens
0.13
-
 UDP-xylose in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 Homo sapiens
0.16
-
 4-methylumbelliferyl-beta-D-xylopyranoside membrane beta1,4-GalT7 in HeLa cells Homo sapiens
0.23
-
 UDP-galactose in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 Homo sapiens
0.27
-
 4-methylumbelliferyl-beta-D-xylopyranoside maltose-binding protein-beta1,4-GalT7 fusion protein Homo sapiens
0.28
-
 UDP-glucose in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 Homo sapiens
0.31
-
 UDP-galactose membrane beta1,4-GalT7 in HeLa cells Homo sapiens
0.85
-
 4-nitrophenyl-beta-D-xylopyranoside membrane beta1,4-GalT7 in HeLa cells Homo sapiens
1.27
-
 4-nitrophenyl-beta-D-xylopyranoside maltose-binding protein-beta1,4-GalT7 fusion protein Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi membrane
-
 Homo sapiens 139
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ enzyme catalyses the Mn2+-dependent transfer of galactose from UDP-galactose the non-reducing end of the xylose residue of the common tetrasaccharide linker region of glycosaminoglycans Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 recombinant fusion protein, by SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-galactose + O-beta-D-xylosylprotein Homo sapiens
-
 UDP + 4-beta-D-galactosyl-O-beta-D-xylosylprotein
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UBV7
-
-

Purification (Commentary)

Purification (Comment) Organism
purification of maltose-binding protein-beta1,4-GalT7 fusion protein with an amylose column Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.058
-
 maltose-binding protein-fusion enzyme, supernatant, in 100 mM cacodylate buffer containing 10 mM MnCl2, pH 7.0 Homo sapiens
0.97
-
 maltose-binding protein-fusion enzyme, amylose elution, in 100 mM cacodylate buffer containing 10 mM MnCl2, pH 7.0 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme is able to transfer glucose, xylose and, to a lesser extent glucuronic acid and N-acetylglucosamine, to acceptor sugars, whereas UDP-mannose and UDP-N-acetyl galactosamine are not substrates Homo sapiens ?
-
 ?
UDP-galactose + O-beta-D-xylosylprotein
-
 Homo sapiens UDP + 4-beta-D-galactosyl-O-beta-D-xylosylprotein
-
 ?
UDP-glucose + 4-methylumbelliferyl-beta-D-xylopyranoside
-
 Homo sapiens ?
-
 ?
UDP-glucuronic acid + 4-methylumbelliferyl-beta-D-xylopyranoside
-
 Homo sapiens ?
-
 ?
UDP-N-acetyl glucosamine + 4-methylumbelliferyl-beta-D-xylopyranoside
-
 Homo sapiens ?
-
 ?
UDP-xylose + 4-methylumbelliferyl-beta-D-xylopyranoside
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
beta1,4-galactosyltransferase 7
-
 Homo sapiens
beta1,4-GalT
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 UDP-glucuronic acid kcat value is above 0.2 1/min in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 Homo sapiens
additional information
-
 UDP-N-acetyl glucosamine kcat value is above 0.2 1/min in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 Homo sapiens
additional information
-
 UDP-mannose kcat value is not detected, in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 Homo sapiens
additional information
-
 UDP-N-acetyl galactosamine kcat value not detected in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 Homo sapiens
3 6 UDP-xylose in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 Homo sapiens
72
-
 UDP-glucose in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 Homo sapiens
4080
-
 4-methylumbelliferyl-beta-D-xylopyranoside recombinant maltose-binding protein-beta1,4-GalT7 fusion protein Homo sapiens
5086
-
 UDP-galactose in 100 mM cacodylate buffer, pH 7.0 containing 10 mM MnCl2 and 0.2-6 microM purified fusion-beta1,4-GalT7 Homo sapiens
5460
-
 4-nitrophenyl-beta-D-xylopyranoside recombinant maltose-binding protein-beta1,4-GalT7 fusion protein Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 CDP Ki value is above 9 mM, UDP-galactose 0.1-16 mM, 0-10 mM CDP Homo sapiens
additional information
-
 UDP-N-acetyl galactosamine Ki value is above 9 mM, UDP-galactose 0.1-16 mM, 0-10 mM UDP-N-acetyl galactosamine Homo sapiens
additional information
-
 UDP-mannose Ki value is above 9, UDP-galactose 0.1-16 mM, 0-10 mM UDP-mannose Homo sapiens
0.61
-
 UDP UDP-galactose 0.1-16 mM, UDP 0-2 mM Homo sapiens
1.65
-
 UTP UDP-galactose 0.1-16 mM, UTP 0-4 mM Homo sapiens
1.93
-
 TDP UDP-galactose 0.1-16 mM, TDP 0-4 mM Homo sapiens

General Information

General Information Comment Organism
physiological function enzyme is involved in the biosynthesis of the tetrasaccharide linker protein region (glucuronic acid beta1,3galactose beta1,3galactose beta1,4xylose beta1) of proteoglycans, by catalysing the transfer of galactose from the uridine 5-diphosphogalactose to a xylose residue, this reaction is rate-limiting in glycosaminoglycan biosynthesis Homo sapiens