Protein Variants | Comment | Organism |
---|---|---|
D203A | mutation has no influence on Alg2 function | Saccharomyces cerevisiae |
D248A | mutation has no influence on Alg2 function | Saccharomyces cerevisiae |
E264A | mutation has no influence on Alg2 function | Saccharomyces cerevisiae |
E335A | mutant has some residual activity | Saccharomyces cerevisiae |
E343A | inactive mutant enzyme | Saccharomyces cerevisiae |
G337A | mutation has no influence on Alg2 function | Saccharomyces cerevisiae |
G337E | nonfunctional enzyme variant | Saccharomyces cerevisiae |
G337R | nonfunctional enzyme variant | Saccharomyces cerevisiae |
G338A | mutation has no influence on Alg2 function | Saccharomyces cerevisiae |
H336A | mutation has no influence on Alg2 function | Saccharomyces cerevisiae |
K206A | mutation has no influence on Alg2 function | Saccharomyces cerevisiae |
K210A | mutation has no influence on Alg2 function | Saccharomyces cerevisiae |
K229A | mutation has no effect on growth and glycosylation | Saccharomyces cerevisiae |
K230A | mutation causes loss of Alg2 activity | Saccharomyces cerevisiae |
K251A | mutation has no influence on Alg2 function | Saccharomyces cerevisiae |
additional information | mutational analysis of Alg2 and identification of amino acids required for its activity. None of the four domains (predicted as transmembrane-spanning helices) is essential for transferase activity because truncated Alg2 variants can exert their function as long as Alg2 is associated with the endaplasmic reticulum by either its N- or C-terminal hydrophobic regions | Saccharomyces cerevisiae |
N231A | mutation has no effect on growth and glycosylation | Saccharomyces cerevisiae |
N392A | mutation has no influence on Alg2 function | Saccharomyces cerevisiae |
P192A | mutation has no influence on Alg2 function | Saccharomyces cerevisiae |
P359A | mutation has no influence on Alg2 function | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Saccharomyces cerevisiae | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-alpha-D-mannose + D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol | Saccharomyces cerevisiae | in eukaryotes, biosynthesis of N-glycans starts with the assembly of the common core oligosaccharide precursor Glc3Man9 GlcNAc2-PP-Dol, the glycan moiety of which is subsequently transferred onto selected Asn-Xaa-(Ser/Thr) acceptor sites of the nascent polypeptide chain by the oligosaccharyl-transferase complex | GDP + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-GlcNAc-diphosphodolichol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P43636 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
no glycoprotein | - |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-alpha-D-mannose + D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol | in eukaryotes, biosynthesis of N-glycans starts with the assembly of the common core oligosaccharide precursor Glc3Man9 GlcNAc2-PP-Dol, the glycan moiety of which is subsequently transferred onto selected Asn-Xaa-(Ser/Thr) acceptor sites of the nascent polypeptide chain by the oligosaccharyl-transferase complex | Saccharomyces cerevisiae | GDP + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-GlcNAc-diphosphodolichol | - |
? | |
GDP-alpha-D-mannose + D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol | Alg2 is able to catalyze both the addition of the alpa1,3- and alpha1,6-linked mannose residue to Man1GlcNAc2-PP-Dol, forming Man2GlcNAc2-PP-Dol (cf EC 2.4.1.132) and subsequently to Man3GlcNAc2-PP-Dol | Saccharomyces cerevisiae | GDP + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-GlcNAc-diphosphodolichol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Alg2 | - |
Saccharomyces cerevisiae |
Alg2 mannosyltransferase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
26 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Saccharomyces cerevisiae |