Any feedback?
Literature summary for 2.4.1.132 extracted from
- The biosynthesis of oligosaccharide-lipids. Activation of mannosyltransferase II by specific phospholipids (1982), J. Biol. Chem., 257, 9025-9029.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cardiolipin | activation, 35% as effective as phosphatidylethanolamine | Oryctolagus cuniculus |  |
| phosphatidylethanolamine | activation | Oryctolagus cuniculus | |
| phosphatidylethanolamine | and mixtures with other phospholipids forming non-bilayer phospholipid phases e.g. hexagonal phases | Oryctolagus cuniculus | |
| phosphatidylethanolamine | no activation by phosphatidylcholine, phosphatidylinositol, phosphatidylglycerol, sphingomyelin, lysophosphocholine or lysophosphoethanolamine | Oryctolagus cuniculus | |
| phosphatidylethanolamine | phosphoethanolamine containing unsaturated acylchains | Oryctolagus cuniculus | |
| phosphatidylethanolamine | in the absence of detergent | Oryctolagus cuniculus | |
| phosphatidylethanolamine | enzyme-phospholipid-complex formation | Oryctolagus cuniculus | |
| phosphatidylethanolamine | from various eukaryotic and prokaryotic sources | Oryctolagus cuniculus | |
| phosphatidylserine | activation, 16-43% as effective as phosphatidylethanolamine | Oryctolagus cuniculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00096 | - |
tetrasaccharide-diphosphoryl-lipid | - |
Oryctolagus cuniculus | |
| 0.00142 | - |
GDPmannose | - |
Oryctolagus cuniculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Oryctolagus cuniculus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Oryctolagus cuniculus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Oryctolagus cuniculus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, in 0.1% v/v Nonidet P-40 and 10% v/v glycerol, at least 3 months | Oryctolagus cuniculus |
| 0-4°C, in 0.1% v/v Nonidet P-40 and 10% v/v glycerol, 24 h | Oryctolagus cuniculus |
| glycerol, 10% v/v, stabilizes during storage | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDPmannose + tetrasaccharide-diphosphoryl-lipid | - |
Oryctolagus cuniculus | GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Oryctolagus cuniculus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
phosphatidylethanolamine increases heat-stability | Oryctolagus cuniculus |
| 37 | - |
5 min, in 0.0225% Nonidet P-40 without substrate, about 80% loss of activity. In phosphatidylethanolamine without substrate: at least 5 min stable, after 25 min about 15% loss of activity | Oryctolagus cuniculus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
