Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Acidithiobacillus caldus |
Protein Variants | Comment | Organism |
---|---|---|
K639R | the mutant exhibits a strongly decreased Km value for UDP compared to the wild type enzyme | Acidithiobacillus caldus |
L636Q/A642N | the mutant exhibits a strongly decreased Km value for UDP compared to the wild type enzyme | Acidithiobacillus caldus |
L636Q/K639R/V641R/A642N | the mutant exhibits a strongly decreased Km value for UDP compared to the wild type enzyme | Acidithiobacillus caldus |
L636Q/L637M/K639R/T640V/V641R/A642N | the mutant exhibits a strongly decreased Km value for UDP and increased Km for ADP as compared to the wild type enzyme | Acidithiobacillus caldus |
L637M | the mutant exhibits a strongly decreased Km value for UDP compared to the wild type enzyme | Acidithiobacillus caldus |
L637M/K639R/T640V | the mutant exhibits a strongly decreased Km value for UDP compared to the wild type enzyme | Acidithiobacillus caldus |
L637M/T640V | the mutant exhibits a strongly decreased Km value for UDP compared to the wild type enzyme | Acidithiobacillus caldus |
T640V | the mutant exhibits a strongly decreased Km value for UDP compared to the wild type enzyme | Acidithiobacillus caldus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
UDP | the wild type enzyme shows no significant inhibition of activity below 20 mM UDP | Acidithiobacillus caldus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
ADP | at pH 7.0 and 60°C | Acidithiobacillus caldus | |
7.8 | - |
UDP | at pH 7.0 and 60°C | Acidithiobacillus caldus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NDP + sucrose | Acidithiobacillus caldus | - |
NDP-alpha-D-glucose + D-fructose | - |
r | |
NDP + sucrose | Acidithiobacillus caldus ATCC 51756 | - |
NDP-alpha-D-glucose + D-fructose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidithiobacillus caldus | A0A059ZV61 | - |
- |
Acidithiobacillus caldus ATCC 51756 | A0A059ZV61 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Acidithiobacillus caldus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + sucrose | - |
Acidithiobacillus caldus | ADP-alpha-D-glucose + D-fructose | - |
r | |
ADP + sucrose | - |
Acidithiobacillus caldus ATCC 51756 | ADP-alpha-D-glucose + D-fructose | - |
r | |
NDP + sucrose | - |
Acidithiobacillus caldus | NDP-alpha-D-glucose + D-fructose | - |
r | |
NDP + sucrose | - |
Acidithiobacillus caldus ATCC 51756 | NDP-alpha-D-glucose + D-fructose | - |
r | |
UDP + sucrose | - |
Acidithiobacillus caldus | UDP-alpha-D-glucose + D-fructose | - |
r | |
UDP + sucrose | - |
Acidithiobacillus caldus ATCC 51756 | UDP-alpha-D-glucose + D-fructose | - |
r |
Synonyms | Comment | Organism |
---|---|---|
SuSy | - |
Acidithiobacillus caldus |