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Literature summary for 2.4.1.128 extracted from
- Glucosylation of the phytoalexin N-feruloyl tyramine modulates the levels of pathogen-responsive metabolites in Nicotiana benthamiana (2019), Plant J., 100, 20-37 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, recombinant overexpression of UGT73A24 in Nicotiana benthamiana leaves by agroinfiltration to identify the natural substrates | Nicotiana benthamiana |
| DNA and amino acid sequence determination and analysis, recombinant overexpression of UGT73A25 in Nicotiana benthamiana leaves by agroinfiltration to identify the natural substrates | Nicotiana benthamiana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | overexpression of UGT73A24 and UGT73A25 leads to a significant reduction in the level of the pathogen-responsive metabolites, including phenylalanine and tryptophan although the latter are not substrates of the encoded enzymes | Nicotiana benthamiana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Nicotiana benthamiana | |
| 0.0022 | - |
kaempferol | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana |  |
| 0.0035 | - |
quercetin | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 0.0043 | - |
quercetin | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 0.0187 | - |
kaempferol | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 0.1103 | - |
N-4-trans-coumaroyl tyramine | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 0.1505 | - |
N-caffeoyl-O-methyl tyramine | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 0.1592 | - |
N-caffeoyl-O-methyl tyramine | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 0.2826 | - |
N-4-trans-coumaroyl tyramine | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 0.3245 | - |
scopoletin | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 0.8818 | - |
carvacrol | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 0.9123 | - |
scopoletin | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 1.2721 | - |
carvacrol | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 1.4732 | - |
N-trans-feruloyl tyramine | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 3.0307 | - |
N-trans-feruloyl tyramine | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Nicotiana benthamiana | UGT73A25 appears to promote the formation of flavonoid rutinosides and a kaempferol diglucoside, while UGT73A24 does not | ? | - |
- |
|
| UDP-glucose + kaempferol | Nicotiana benthamiana | best substrate | UDP + ? | - |
? | |
| UDP-glucose + kaempferol | Nicotiana benthamiana | - |
UDP + kaempferol 3,7-diglucoside | - |
? | |
| UDP-glucose + N-trans-feruloyl tyramine | Nicotiana benthamiana | - |
UDP + ? | - |
? | |
| UDP-glucose + scopoletin | Nicotiana benthamiana | - |
UDP + scopolin | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nicotiana benthamiana | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| callus | - |
Nicotiana benthamiana | - |
| leaf | - |
Nicotiana benthamiana | - |
| root | - |
Nicotiana benthamiana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | UGT73A25 appears to promote the formation of flavonoid rutinosides and a kaempferol diglucoside, while UGT73A24 does not | Nicotiana benthamiana | ? | - |
- |
|
| additional information | broad substrate specificity of NbUGT73A25, detailed overview. Enzymes UGT73A24 and UGT73A25 show the highest activity towards kaempferol | Nicotiana benthamiana | ? | - |
- |
|
| UDP-glucose + carvacrol | - |
Nicotiana benthamiana | UDP + carvacrol | - |
? | |
| UDP-glucose + kaempferol | best substrate | Nicotiana benthamiana | UDP + ? | - |
? | |
| UDP-glucose + kaempferol | - |
Nicotiana benthamiana | UDP + kaempferol 3,7-diglucoside | - |
? | |
| UDP-glucose + kaempferol | best substrate | Nicotiana benthamiana | UDP + kaempferol 3,7-diglucoside | - |
? | |
| UDP-glucose + N-4-trans-coumaroyl tyramine | - |
Nicotiana benthamiana | UDP + ? | - |
? | |
| UDP-glucose + N-caffeoyl-O-methyl tyramine | - |
Nicotiana benthamiana | UDP + ? | - |
? | |
| UDP-glucose + N-trans-feruloyl tyramine | - |
Nicotiana benthamiana | UDP + ? | - |
? | |
| UDP-glucose + quercetin | - |
Nicotiana benthamiana | UDP + ? | - |
? | |
| UDP-glucose + scopoletin | - |
Nicotiana benthamiana | UDP + scopolin | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| N-feruloyltyramine glucosyltransferase | - |
Nicotiana benthamiana |
| NbUGT73A24 | - |
Nicotiana benthamiana |
| NbUGT73A25 | - |
Nicotiana benthamiana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Nicotiana benthamiana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
N-4-trans-coumaroyl tyramine | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 0.06 | - |
N-caffeoyl-O-methyl tyramine | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 0.06 | - |
N-trans-feruloyl tyramine | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 0.07 | - |
N-caffeoyl-O-methyl tyramine | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 0.264 | - |
quercetin | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 0.341 | - |
kaempferol | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 0.589 | - |
kaempferol | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 0.65 | - |
quercetin | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 0.766 | - |
scopoletin | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 0.811 | - |
carvacrol | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 0.916 | - |
scopoletin | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 0.967 | - |
carvacrol | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Nicotiana benthamiana |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | Nicotiana benthamiana enzymes UGT73A24 and UGT73A25 show 95% sequence identity | Nicotiana benthamiana |
| metabolism | while in Nicotiana tabacum, TOGT (tobacco UGT) glucosylates scopoletin after treatment with salicylate, fungal elicitors and the tobacco mosaic virus, enzymes NbUGT73A24 and NbUGT73A25 produce glucosides of phytoalexin N-feruloyl tyramine, which may strengthen cell walls to prevent the intrusion of pathogens, and flavonols after agroinfiltration of the corresponding genes in Nicotiana benthamiana | Nicotiana benthamiana |
| physiological function | NbUGT73A24 produces glucosides of phytoalexin N-feruloyl tyramine, which may strengthen cell walls to prevent the intrusion of pathogens, and flavonols after agroinfiltration of the corresponding genes in Nicotiana benthamiana | Nicotiana benthamiana |
| physiological function | NbUGT73A25 produces glucosides of phytoalexin N-feruloyl tyramine, which may strengthen cell walls to prevent the intrusion of pathogens, and flavonols after agroinfiltration of the corresponding genes in Nicotiana benthamiana | Nicotiana benthamiana |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
N-trans-feruloyl tyramine | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 0.035 | - |
N-4-trans-coumaroyl tyramine | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 0.041 | - |
N-trans-feruloyl tyramine | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 0.09 | - |
N-4-trans-coumaroyl tyramine | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 0.4 | - |
N-caffeoyl-O-methyl tyramine | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 0.44 | - |
N-caffeoyl-O-methyl tyramine | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 0.76 | - |
carvacrol | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 0.84 | - |
scopoletin | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 0.92 | - |
carvacrol | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 2.82 | - |
scopoletin | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 31.5 | - |
kaempferol | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 61.4 | - |
quercetin | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 155 | - |
kaempferol | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
| 185.71 | - |
quercetin | recombinant enzyme, pH 7.5, 30°C | Nicotiana benthamiana | |
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