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Literature summary for 2.4.1.123 extracted from
- Differential expression of two galactinol synthase isoforms LcGolS1 and LcGolS2 in developing lentil (Lens culinaris Medik. cv CDC Redberry) seeds (2016), Plant Physiol. Biochem., 108, 422-433 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| LcGolS1, cDNA library construction with RNA isolated from developing lentil seeds, and screening, DNA and amino acid sequence determination and analysis, sequence comparison with isozyme LcGolS2, phylogenetic analysis, and quantitative real time PCR expression analysis | Lens culinaris |
| LcGolS2, cDNA library construction with RNA isolated from developing lentil seeds, and screening, DNA and amino acid sequence determination and analysis, sequence comparison with isozyme LcGolS1, phylogenetic analysis, and quantitative real time PCR expression analysis | Lens culinaris |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-galactose + myo-inositol | Lens culinaris | - |
UDP + O-alpha-D-galactosyl-(1->3)-1D-myo-inositol | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lens culinaris | A0A0S2I591 | i.e. Cicer lens, cv. CDC Redberry | - |
| Lens culinaris | A0A0S2I5F8 | i.e. Cicer lens, cv. CDC Redberry | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | enzyme activity of GS correlates with GolS transcript accumulation and galactinol accumulation | Lens culinaris | - |
| seed | during lentil seed development, isozyme LcGolS1 transcripts show higher accumulation during 26-32 days after flowering (DAF) corresponding to seed desiccation, while isozyme LcGolS2 shows maximum accumulation at 24 DAF, prior to increase in LcGolS1 transcripts | Lens culinaris | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-galactose + myo-inositol | - |
Lens culinaris | UDP + O-alpha-D-galactosyl-(1->3)-1D-myo-inositol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37600, about, sequence calculation | Lens culinaris |
| ? | x * 38700, about, sequence calculation | Lens culinaris |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| galactinol synthase | - |
Lens culinaris |
| LcGolS1 | - |
Lens culinaris |
| LcGolS2 | - |
Lens culinaris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Lens culinaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Lens culinaris |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | in-silico protein structure prediction of LcGolS1 | Lens culinaris |
| additional information | in-silico protein structure prediction of LcGolS2 | Lens culinaris |
| physiological function | galactinol synthase catalyzes the transfer of a galactosyl residue from UDP-galactose to myo-inositol to synthesize galactinol, a precursor for raffinose family oligosaccharides (RFO) biosynthesis. Accumulation of raffinose family oligosaccharides (RFO), galactinol and its precursors during seed development, overview | Lens culinaris |
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Release 2023.1 (January 2023)
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