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Literature summary for 2.4.1.122 extracted from
- Glucuronylated core 1 glycans are required for precise localization of neuromuscular junctions and normal formation of basement membranes on Drosophila muscles (2018), Dev. Biol., 436, 108-124 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Cosmc (also known as C1GALT1C1), an endoplasmic reticulum chaperone, is essential for the precise folding and activity of C1GalT1 in mammals. In contrast to mammalian orthologues, Drosophila dC1GalT1 does not require a chaperone such as Cosmc | Drosophila melanogaster |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| genetic interaction between dGlcAT-P and dC1GalT1 | Drosophila melanogaster |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | generation of dC1GalT12.1/+:dGlcAT-PSK6/+ double heterozygous mutants, phenotypes, overview | Drosophila melanogaster |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| basement membrane | a specialized extracellular matrix that underlies the epithelium and surrounds other tissues, including muscles, fat, and nerve fibers | Drosophila melanogaster | 5604 | - |
| neuromuscular junction | NMJ | Drosophila melanogaster | 31594 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-galactose + N-acetyl-alpha-D-galactosaminyl-R | Drosophila melanogaster | - |
UDP + beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | Q7K237 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| central nervous system | - |
Drosophila melanogaster | - |
| hemocyte | - |
Drosophila melanogaster | - |
| larva | - |
Drosophila melanogaster | - |
| lymph node | - |
Drosophila melanogaster | - |
| additional information | T antigen produced by Drosophila C1GalT1 (dC1GalT1) is expressed in various tissues | Drosophila melanogaster | - |
| muscle fibre | - |
Drosophila melanogaster | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | analysis of glycan structure by mass spectrometry | Drosophila melanogaster | ? | - |
- |
|
| UDP-alpha-D-galactose + Galbeta1-3GalNAcalpha1-4-nitrophenol | - |
Drosophila melanogaster | ? | - |
? | |
| UDP-alpha-D-galactose + N-acetyl-alpha-D-galactosaminyl-R | - |
Drosophila melanogaster | UDP + beta-D-galactosyl-(1->3)-N-acetyl-alpha-D-galactosaminyl-R | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| C1GALT1 | - |
Drosophila melanogaster |
| C1GalTA | - |
Drosophila melanogaster |
| core 1 beta1,3-galactosyltrasferase 1 | - |
Drosophila melanogaster |
| dC1GalT1 | - |
Drosophila melanogaster |
| glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1 | UniProt | Drosophila melanogaster |
| T-synthase | - |
Drosophila melanogaster |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Drosophila melanogaster |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
assay at | Drosophila melanogaster |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | dC1GalT1 loss in larvae leads to various defects, including a decreased number of circulating hemocytes, hyperdifferentiation of hematopoietic stem cells in lymph glands, malformation of the central nervous system, mislocalization of neuromuscular junction (NMJ) boutons, and ultrastructural abnormalities in NMJs and muscle cells. Mutant larvae show lower expression of glucuronylated T antigen on the muscles and at NMJs. Mislocalization of neuromuscular junction (NMJ) boutons and a partial loss of the basement membrane components collagen IV (Col IV) and nidogen (Ndg) at the muscle 6/7 boundary are observed. Those two phenotypes are correlated and identical to previously described phenotypes in dC1GalT1 mutant larvae | Drosophila melanogaster |
| metabolism | dGlcAT-P (EC 2.4.1.135) genetically interactes with dC1GalT1, and glucuronylated T antigen, rather than unmodified T antigen, contributes to precise localization of NMJ boutons and normal formation of basement membranes on muscles 6/7 | Drosophila melanogaster |
| physiological function | the major components of basement membrane are proteins modified by glycans. T antigen (Galbeta1-3GalNAcalpha1-Ser/Thr) is an evolutionary-conserved mucin-type core 1 glycan structure in animals synthesized by core 1 beta1,3-galactosyltrasferase 1 (C1GalT1). T antigen produced by Drosophila C1GalT1 (dC1GalT1) is expressed in various tissues. T antigen formation occurs mainly due to the activity of Drosophila C1GalT1 orthologue | Drosophila melanogaster |
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