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Literature summary for 2.4.1.121 extracted from
- Indole-3-acetic acid UDP-glucosyltransferase from immature seeds of pea is involved in modification of glycoproteins (2015), Phytochemistry, 117, 25-33.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-gluconic acid lactone | the inhibitor of beta-glucosidase activity, potentiates the enzyme activity at the optimal concentration of 0.3 mM | Pisum sativum |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-naphthalene acetic acid | competitive inhibitor | Pisum sativum | |
| indole-3-butyric acid | competitive inhibitor | Pisum sativum | |
| Indole-3-propionic acid | competitive inhibitor | Pisum sativum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.52 | - |
(indol-3-yl)acetate | pH 7.6, 30°C | Pisum sativum | |
| 1.5 | - |
(indol-3-yl)propionate | pH 7.6, 30°C | Pisum sativum | |
| 2.67 | - |
UDP-glucose | pH 7.6, 30°C | Pisum sativum | |
| 4 | - |
1-naphthalene acetate | pH 7.6, 30°C | Pisum sativum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Pisum sativum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + (indol-3-yl)acetate | Pisum sativum | - |
UDP + 1-O-(indol-3-yl)acetyl-beta-D-glucose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from immature seeds by PEG fractionation, anion exchange chromatography, and preparative PAGE | Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | immature | Pisum sativum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.06 | - |
purified enzyme, substrate gibberellin GA3, pH 7.6, 30°C | Pisum sativum |
| 0.656 | - |
purified enzyme, substrate (indol-3-yl)propionate, pH 7.6, 30°C | Pisum sativum |
| 0.715 | - |
purified enzyme, substrate 1-naphthalene acetate, pH 7.6, 30°C | Pisum sativum |
| 2.976 | - |
purified enzyme, substrate (indol-3-yl)acetate, pH 7.6, 30°C | Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-glucose + (indol-3-yl)acetate | - |
Pisum sativum | UDP + 1-O-(indol-3-yl)acetyl-beta-D-glucose | - |
r | |
| UDP-glucose + (indol-3-yl)acetate | best substrate | Pisum sativum | UDP + 1-O-(indol-3-yl)acetyl-beta-D-glucose | - |
r | |
| UDP-glucose + (indol-3-yl)propionate | 22% of the activity with (indol-3-yl)acetate | Pisum sativum | UDP + 1-O-(indol-3-yl)propionyl-beta-D-glucose | - |
r | |
| UDP-glucose + 1-naphthalene acetate | 24% of the activity with (indol-3-yl)acetate | Pisum sativum | UDP + 1-naphthalene acetyl-beta-D-glucose | - |
r | |
| UDP-glucose + gibberellin GA3 | 1.9% of the activity with (indol-3-yl)acetate | Pisum sativum | UDP + ? | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| IAA glucosyltransferase | - |
Pisum sativum |
| IAGlc synthase | - |
Pisum sativum |
| indole-3-acetic acid UDP-glucosyltransferase | - |
Pisum sativum |
| UDPG: indole-3-ylacetyl-beta-D-glucosyltransferase | - |
Pisum sativum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Pisum sativum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.49 | - |
UDP-glucose | pH 7.6, 30°C | Pisum sativum | |
| 0.53 | - |
1-naphthalene acetate | pH 7.6, 30°C | Pisum sativum | |
| 0.72 | - |
(indol-3-yl)propionate | pH 7.6, 30°C | Pisum sativum | |
| 2.27 | - |
(indol-3-yl)acetate | pH 7.6, 30°C | Pisum sativum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
- |
Pisum sativum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.038 | - |
2-naphthalene acetic acid | pH 7.6, 30°C | Pisum sativum | |
| 0.1 | - |
indole-3-butyric acid | pH 7.6, 30°C | Pisum sativum | |
| 0.15 | - |
Indole-3-propionic acid | pH 7.6, 30°C | Pisum sativum | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the UDP-sugar dependent transferases | Pisum sativum |
| metabolism | the enzyme catalyzes the first step in the biosynthesis of indol-3-yl ester conjugates in monocotyledonous plants | Pisum sativum |
| physiological function | 1-O-(indol-3-yl)acetyl-beta-D-glucose, synthesized by the enzyme, is the substrate for (indol-3-yl)acetate labeling of glycoproteins from pea seeds indicating a possible role of this enzyme in the covalent modification of a class of proteins by a plant hormone. Auxin is a key plant hormone that regulates many physiological processes in plants including organ morphogenesis (i.e. apical dominance, root development) and environmental responses (biotic and abiotic stress). The major auxin is indole-3-acetic acid (IAA), but other indole-derived carboxylic acids, i.e. indole-3-butyric acid, indole-3-propionic acid, 4-chloro-indole-3-acetic acid, and indole-3-pyruvic acid have also auxin activity. Auxin action is highly dependent on its concentration, which must be precisely controlled | Pisum sativum |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
1-naphthalene acetate | pH 7.6, 30°C | Pisum sativum | |
| 0.16 | - |
UDP-glucose | pH 7.6, 30°C | Pisum sativum | |
| 0.46 | - |
(indol-3-yl)propionate | pH 7.6, 30°C | Pisum sativum | |
| 4.36 | - |
(indol-3-yl)acetate | pH 7.6, 30°C | Pisum sativum | |
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