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Literature summary for 2.4.1.121 extracted from
- Enzymic synthesis of indole-3-acetyl-1-O-beta-D-glucose (1988), Plant Physiol., 88, 1474-1480.
General Stability
| General Stability | Organism |
|---|---|
| loses activity during column chromatographic procedures | Zea mays |
| retention of 79% of the initial activity after 16 h dialysis | Zea mays |
| stable to lyophilization | Zea mays |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | 100 mM phosphate buffer, 50% inhibition | Zea mays |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46500 | - |
gel filtration | Zea mays |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDPglucose + indole-3-acetate | Zea mays | first enzyme-catalyzed reaction leading from indole-3-acetic acid to the myo-inositol ester of indole-3-acetate | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Zea mays |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| endosperm | - |
Zea mays | - |
| kernel | - |
Zea mays | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.798 | - |
- |
Zea mays |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for 15 d, 15% loss of activity after 35 d | Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDPglucose + indole-3-acetate | - |
Zea mays | UDP + indole-3-acetyl-beta-1-D-glucose | - |
? | |
| UDPglucose + indole-3-acetate | first enzyme-catalyzed reaction leading from indole-3-acetic acid to the myo-inositol ester of indole-3-acetate | Zea mays | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | 7.6 | - |
Zea mays |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.7 | 7.6 | pH 7.3-7.6: maximal activity, no activity below pH 6.7 | Zea mays |
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Release 2023.1 (January 2023)
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