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Literature summary for 2.4.1.120 extracted from

  • Kajla, S.; Mukhopadhyay, A.; Pradhan, A.
    Development of transgenic Brassica juncea lines for reduced seed sinapine content by perturbing phenylpropanoid pathway genes (2017), PLoS ONE, 12, e0182747 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene SGT, recombinant expression of full-length enzyme in Brassica juncea via Agrobacterium tumefaciens mediated transformation Brassica juncea

Protein Variants

Protein Variants Comment Organism
additional information two genes are isolated from Brassica juncea and eight silencing constructs are developed using three different RNA silencing approaches viz. antisense RNA, RNAi and artificial microRNA. Transgenics in Brassica juncea are developed following Agrobacterium-mediated transformation. From a total of 1232 independent T0 transgenic events obtained using eight silencing constructs, 25 homozygous lines showing single gene inheritance are identified in the T2 generation. Reduction of seed sinapine content in these lines range from 15.8% to 67.2% compared to wild-type. Development of suppression constructs for SGT, development of silencing constructs for SGT genes viz. antisense RNA (BjSGTAS for SGT), RNAi (BjSGTRNAi for SGT), and artificial microRNA (BjSGTamiR38 and BjSGTamiR40 for SGT), genetic analysis of T2 transgenics and establishment of homozygous lines Brassica juncea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + sinapate Brassica juncea
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UDP + 1-O-sinapoyl-beta-D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Brassica juncea A0A0M3PZA4 cv. Varuna
-

Source Tissue

Source Tissue Comment Organism Textmining
seed expression analysis of SGT gene measured at different stages of seed development Brassica juncea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + sinapate
-
Brassica juncea UDP + 1-O-sinapoyl-beta-D-glucose
-
?

Synonyms

Synonyms Comment Organism
BjSGT
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Brassica juncea
SGT
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Brassica juncea
SGT enzyme
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Brassica juncea
UDP-glucose: sinapate glucosyltransferase
-
Brassica juncea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Brassica juncea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Brassica juncea

General Information

General Information Comment Organism
metabolism SGT and SCT, encoding enzymes UDP-glucose: sinapate glucosyltransferase and sinapoylglucose: choline sinapoyltransferase, respectively, are involved in the final two steps of sinapine biosynthetic pathway. Comparison of the two enzymes, overview Brassica juncea
additional information in-silico structural analysis and molecular docking. Three dimensional structure and binding mode of sinapate with SGT enzyme, overview Brassica juncea