Cloned (Comment) | Organism |
---|---|
gene SGT, recombinant expression of full-length enzyme in Brassica juncea via Agrobacterium tumefaciens mediated transformation | Brassica juncea |
Protein Variants | Comment | Organism |
---|---|---|
additional information | two genes are isolated from Brassica juncea and eight silencing constructs are developed using three different RNA silencing approaches viz. antisense RNA, RNAi and artificial microRNA. Transgenics in Brassica juncea are developed following Agrobacterium-mediated transformation. From a total of 1232 independent T0 transgenic events obtained using eight silencing constructs, 25 homozygous lines showing single gene inheritance are identified in the T2 generation. Reduction of seed sinapine content in these lines range from 15.8% to 67.2% compared to wild-type. Development of suppression constructs for SGT, development of silencing constructs for SGT genes viz. antisense RNA (BjSGTAS for SGT), RNAi (BjSGTRNAi for SGT), and artificial microRNA (BjSGTamiR38 and BjSGTamiR40 for SGT), genetic analysis of T2 transgenics and establishment of homozygous lines | Brassica juncea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + sinapate | Brassica juncea | - |
UDP + 1-O-sinapoyl-beta-D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica juncea | A0A0M3PZA4 | cv. Varuna | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | expression analysis of SGT gene measured at different stages of seed development | Brassica juncea | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + sinapate | - |
Brassica juncea | UDP + 1-O-sinapoyl-beta-D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BjSGT | - |
Brassica juncea |
SGT | - |
Brassica juncea |
SGT enzyme | - |
Brassica juncea |
UDP-glucose: sinapate glucosyltransferase | - |
Brassica juncea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Brassica juncea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Brassica juncea |
General Information | Comment | Organism |
---|---|---|
metabolism | SGT and SCT, encoding enzymes UDP-glucose: sinapate glucosyltransferase and sinapoylglucose: choline sinapoyltransferase, respectively, are involved in the final two steps of sinapine biosynthetic pathway. Comparison of the two enzymes, overview | Brassica juncea |
additional information | in-silico structural analysis and molecular docking. Three dimensional structure and binding mode of sinapate with SGT enzyme, overview | Brassica juncea |