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Literature summary for 2.4.1.120 extracted from
- Identification and characterization of DcUSAGT1, a UDP-glucose sinapic acid glucosyltransferase from purple carrot taproots (2016), PLoS ONE, 11, e0154938 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene DcUSAGT1, sequence comparisons, the expression levels of DcUSAGT1 gene in the purple carrot taproots may be higher than those in non-purple carrot taproots, quantitative real-time PCR expression analysis, recombinant expression of His-tagged DcUSAGT1 in Escherichia coli strain BL21(DE3) | Daucus carota |
| gene DcUSAGT1, sequence comparisons, the expression levels of DcUSAGT1 gene in the purple carrot taproots may be higher than those in non-purple carrot taproots, quantitative real-time PCR expression analysis, recombinant expression of His-tagged DcUSAGT1 in Escherichia coli strain BL21(DE3) | Daucus carota subsp. sativus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.59 | - |
sinapate | pH 7.0, 30°C, recombinant enzyme | Daucus carota |  |
| 0.59 | - |
sinapate | pH 7.0, 30°C, recombinant enzyme | Daucus carota subsp. sativus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + sinapate | Daucus carota | - |
UDP + 1-O-sinapoyl-beta-D-glucose | - |
? | |
| UDP-alpha-D-glucose + sinapate | Daucus carota subsp. sativus | - |
UDP + 1-O-sinapoyl-beta-D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Daucus carota | - |
non-purple carrot cultivars Kurodagosun, Sanhongliucun, and Junchuanhong | - |
| Daucus carota subsp. sativus | - |
var. atrorubens, purple carrot cultivars Deep purple, Purple68, and Tianzi2-hao | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged DcUSAGT1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and desalting gel filtration | Daucus carota |
| recombinant His-tagged DcUSAGT1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and desalting gel filtration | Daucus carota subsp. sativus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | crude enzyme extracted from the purple carrot taproots rather than non-purple carrot taproots exhibits USAGT activity. Expression profiles of the enzyme in the three non-purple carrot cultivars Kurodagosun, Sanhongliucun, and Junchuanhong, quantitative real-time PCR expression analysis, overview | Daucus carota | - |
| root | crude enzyme extracted from the purple carrot taproots rather than non-purple carrot taproots exhibits USAGT activity. Expression profiles of the enzyme in the three purple carrot cultivars Deep purple, Purple68, and Tianzi2-hao, quantitative real-time PCR expression analysis, overview | Daucus carota subsp. sativus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + sinapate | - |
Daucus carota | UDP + 1-O-sinapoyl-beta-D-glucose | - |
? | |
| UDP-alpha-D-glucose + sinapate | - |
Daucus carota subsp. sativus | UDP + 1-O-sinapoyl-beta-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 54550, sequence calculation | Daucus carota |
| ? | x * 54550, sequence calculation | Daucus carota subsp. sativus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DcUSAGT1 | - |
Daucus carota |
| DcUSAGT1 | - |
Daucus carota subsp. sativus |
| UDP-glucose: sinapic acid glucosyltransferase | - |
Daucus carota |
| UDP-glucose: sinapic acid glucosyltransferase | - |
Daucus carota subsp. sativus |
| USAGT | - |
Daucus carota |
| USAGT | - |
Daucus carota subsp. sativus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
recombinant enzyme | Daucus carota |
| 30 | - |
recombinant enzyme | Daucus carota subsp. sativus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 40 | over 40% of maximal activity at 20-40°C, inactivation at 50°C | Daucus carota |
| 20 | 40 | over 40% of maximal activity at 20-40°C, inactivation at 50°C | Daucus carota subsp. sativus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
sinapate | pH 7.0, 30°C, recombinant enzyme | Daucus carota | |
| 0.04 | - |
sinapate | pH 7.0, 30°C, recombinant enzyme | Daucus carota subsp. sativus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
recombinant enzyme | Daucus carota |
| 7 | - |
recombinant enzyme | Daucus carota subsp. sativus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | over 70% of maximal activity at pH 6.0-7.5, 30% at pH 8.5, inactive at pH 9.0 | Daucus carota |
| 6 | 8.5 | over 70% of maximal activity at pH 6.0-7.5, 30% at pH 8.5, inactive at pH 9.0 | Daucus carota subsp. sativus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the glycosyltransferase-B type superfamily | Daucus carota |
| evolution | the enzyme belongs to the glycosyltransferase-B type superfamily | Daucus carota subsp. sativus |
| metabolism | in the 1-O-sinapoylglucose biosynthesis pathway, Sinapic acid and UDP-glucose act as potential substrates for the UDP-glucose: sinapic acid glucosyltransferase, which catalyzes the transfer of glucose moiety from UDP-glucose to the 1-O-position of sinapic acid | Daucus carota |
| metabolism | in the 1-O-sinapoylglucose biosynthesis pathway, Sinapic acid and UDP-glucose act as potential substrates for the UDP-glucose: sinapic acid glucosyltransferase, which catalyzes the transfer of glucose moiety from UDP-glucose to the 1-O-position of sinapic acid | Daucus carota subsp. sativus |
| physiological function | UDP-glucose: sinapic acid glucosyltransferase (USAGT1) from Daucus carota may influence anthocyanin biosynthesis of purple carrot taproots, DcUSAGT1 may play important roles in the stability of anthocyanin accumulation | Daucus carota |
| physiological function | UDP-glucose: sinapic acid glucosyltransferase (USAGT1) from Daucus carota may influence anthocyanin biosynthesis of purple carrot taproots, DcUSAGT1 may play important roles in the stability of anthocyanin accumulation | Daucus carota subsp. sativus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.068 | - |
sinapate | pH 7.0, 30°C, recombinant enzyme | Daucus carota | |
| 0.068 | - |
sinapate | pH 7.0, 30°C, recombinant enzyme | Daucus carota subsp. sativus | |
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