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Literature summary for 2.4.1.120 extracted from

  • Milkowski, C.; Baumert, A.; Schmidt, D.; Nehlin, L.; Strack, D.
    Molecular regulation of sinapate ester metabolism in Brassica napus: expression of genes, properties of the encoded proteins and correlation of enzyme activities with metabolite accumulation (2004), Plant J., 38, 80-92.
    View publication on PubMed
Search for more literature for 2.4.1.120

Cloned(Commentary)

Cloned (Comment) Organism
genomic organization analysis, expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Brassica napus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic data for unpurified enzyme in crude protein extract of Escherichia coli expressing the recombinant enzyme Brassica napus
0.72
-
 sinapate
-
 Brassica napus
1
-
 UDP-glucose
-
 Brassica napus
1.7
-
 ferulate
-
 Brassica napus
2.3
-
 caffeate
-
 Brassica napus
3.6
-
 4-coumarate
-
 Brassica napus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50270
-
 x * 55970, unmodified enzyme, amino acid sequence calculation, x * 50270, mature enzyme, amino acid sequence calculation Brassica napus
55970
-
 x * 55970, unmodified enzyme, amino acid sequence calculation, x * 50270, mature enzyme, amino acid sequence calculation Brassica napus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + sinapate Brassica napus regulation of the sinapate ester metabolism, enzyme expression is restricted to developing seeds, where it is involved in accumulation kinetics of corresponding metabolites UDP + 1-O-sinapoyl-beta-D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Brassica napus Q9FYU7
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein enzyme is putatively N-glycosylated in the Golgi apparatus Brassica napus
proteolytic modification enzyme contains a signal peptide, cleavage probably after residue D19 Brassica napus

Reaction

Reaction Comment Organism Reaction ID
UDP-alpha-D-glucose + sinapate = UDP + 1-O-sinapoyl-beta-D-glucose random bi bi mechanism Brassica napus
a

Source Tissue

Source Tissue Comment Organism Textmining
additional information expression analysis during seed development, accumulation of BnSGT1 transcripts and sinapate esters at early cotyledonary stages, overview Brassica napus
-
seed transcription levels increase throughout early stages of seed development until the early- and mid-cotyledonary stage, and stays constant in later stages Brassica napus
-
seedling highest expression level at age of 2 days, followed by a dramatic decrease Brassica napus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + 4-coumarate recombinant enzyme, 2% efficiency compared to sinapate Brassica napus UDP + 1-O-(4-coumaroyl)-beta-D-glucose
-
 ?
UDP-alpha-D-glucose + ferulate recombinant enzyme, 4% efficiency compared to sinapate Brassica napus UDP + 1-O-feruloyl-beta-D-glucose
-
 ?
UDP-alpha-D-glucose + sinapate regulation of the sinapate ester metabolism, enzyme expression is restricted to developing seeds, where it is involved in accumulation kinetics of corresponding metabolites Brassica napus UDP + 1-O-sinapoyl-beta-D-glucose
-
 ?
UDP-alpha-D-glucose + sinapate recombinant enzyme, best substrate Brassica napus UDP + 1-O-sinapoyl-beta-D-glucose
-
 ?
UDP-glucose + caffeate recombinant enzyme, 1% efficiency compared to sinapate Brassica napus UDP + 1-caffeoyl-D-glucose
-
 ?

Subunits

Subunits Comment Organism
? x * 55970, unmodified enzyme, amino acid sequence calculation, x * 50270, mature enzyme, amino acid sequence calculation Brassica napus

Synonyms

Synonyms Comment Organism
BnSGT1
-
 Brassica napus
More enzyme belongs to the serine carboxypeptidase-like family of acyltransferases Brassica napus
UDP-Glc:B. napus sinapate glucosyltransferase
-
 Brassica napus
UDP-glucose:B. napus sinapate glucosyltransferase
-
 Brassica napus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Brassica napus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Brassica napus

pI Value

Organism Comment pI Value Maximum pI Value
Brassica napus sequence calculation
-
 6.1
Brassica napus sequence calculation, mature protein
-
 6.6