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Literature summary for 2.4.1.120 extracted from

  • Strack, D.
    Enzymatic synthesis of 1-sinapoylglucose from free sinapic acid and UDP-glucose by a cell-free system from Raphanus saticus seedlings (1979), Z. Naturforsch. C, 35, 204-208.
No PubMed abstract available
Search for more literature for 2.4.1.120

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol stimulation Raphanus sativus
dithiothreitol 10 mM, 3fold stimulation Raphanus sativus
additional information SH-group required Raphanus sativus

Inhibitors

Inhibitors Comment Organism Structure
PCMB 100% inhibition at 1 mM, activity can be restored up to 50% by addition of 10 mM dithiothreitol, 24% inhibition at 0.1 mM, activity can be completely restored by 10 mM dithiothreitol Raphanus sativus
UDP strong Raphanus sativus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + sinapic acid Raphanus sativus detoxification of sinapic acid and activation of sinapic acid for the subsequent sinapoyltransferase reaction leading to sinapoylmalate UDP + 1-O-sinapoyl-beta-D-glucose
-
 r

Organism

Organism UniProt Comment Textmining
Raphanus sativus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Raphanus sativus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + 3,5-dihydroxybenzoic acid 5% of activity compared to sinapic acid Raphanus sativus UDP + 1-O-(3,5-dihydroxybenzoyl)-beta-D-glucose
-
 ?
UDP-alpha-D-glucose + 4-coumaric acid 40% of activity compared to sinapic acid Raphanus sativus UDP + 1-O-(4-coumaroyl)-beta-D-glucose
-
 ?
UDP-alpha-D-glucose + 4-hydroxybenzoic acid 31% of activity compared to sinapic acid Raphanus sativus UDP + 1-O-(4-hydroxybenzoyl)-beta-D-glucose
-
 ?
UDP-alpha-D-glucose + anthranilic acid 31% of activity compared to sinapic acid Raphanus sativus UDP + 1-O-anthraniloyl-beta-D-glucose
-
 ?
UDP-alpha-D-glucose + benzoic acid 39% of activity compared to sinapic acid Raphanus sativus UDP + 1-O-benzoyl-beta-D-glucose
-
 ?
UDP-alpha-D-glucose + caffeic acid 38% of activity compared to sinapic acid Raphanus sativus UDP + 1-O-caffeoyl-beta-D-glucose
-
 ?
UDP-alpha-D-glucose + cinnamic acid 16% of activity compared to sinapic acid Raphanus sativus UDP + 1-O-cinnamoyl-beta-D-glucose
-
 ?
UDP-alpha-D-glucose + sinapic acid
-
 Raphanus sativus UDP + 1-O-sinapoyl-beta-D-glucose
-
 r
UDP-alpha-D-glucose + sinapic acid detoxification of sinapic acid and activation of sinapic acid for the subsequent sinapoyltransferase reaction leading to sinapoylmalate Raphanus sativus UDP + 1-O-sinapoyl-beta-D-glucose
-
 r
UDP-alpha-D-glucose + syringic acid 35% of activity compared to sinapic acid Raphanus sativus UDP + 1-O-syringoyl-beta-D-glucose
-
 ?
UDP-alpha-D-glucose + vanillic acid 36% of activity compared to sinapic acid Raphanus sativus UDP + 1-O-vanilloyl-beta-D-glucose
-
 ?
UDPalpha-D-glucose + ferulic acid 38% of activity compared to sinapic acid Raphanus sativus UDP + 1-O-feruloyl-beta-D-glucose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Raphanus sativus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 45 25°C: 50% of maximal activity, 45°C: 80% of maximal activity Raphanus sativus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Raphanus sativus