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Literature summary for 2.4.1.12 extracted from

  • Atanassov, I.; Pittman, J.; Turner, S.
    Elucidating the mechanisms of assembly and subunit interaction of the cellulose synthase complex of Arabidopsis secondary cell walls (2009), J. Biol. Chem., 284, 3833-3841.
    View publication on PubMed
Search for more literature for 2.4.1.12

Cloned(Commentary)

Cloned (Comment) Organism
expression of His- and STREP-tagged CESA4, CESA7, and CESA8 in Arabidopsis thaliana using the Agrobacterium tumefaciens transfection nethod in the mutant lines are irx1-1, irx3-1, and irx5-2 Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information the naturally occuring irx3-1 and irx5-2 mutations are caused by premature stop codons that result in protein truncation of CESA7 and CESA4, respectively. In the naturally occuring irx3-1 background, interaction between CESA4 and CESA8 is greatly reduced, and the proteins fail to localize to the plasma membrane. Cellulose contents of mutant lines irx5 and irx1 plants recombinantly expressing CESA8 and CESA4, irx5-2:STREP-CESA4 and irx1-1:STREPCESA8, are much lower than wild-type and as such are not fully functional. Recombinant mutant irx3-1 plants expressing STREP-CESA7 and His/FLAGCESA7 in a dual tag system have wild-type cellulose content and xylem phenotypes, overview. irx3-1:STREP-CESA7-irx3-1:His/FLAG-CESA7 crossed plants phenotype, overview Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cell wall secondary Arabidopsis thaliana 5618
-
microsome
-
 Arabidopsis thaliana
-
-
plasma membrane
-
 Arabidopsis thaliana 5886
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Arabidopsis thaliana cellulose synthase A is a catalytic component of the cellulose synthase complex ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
 Landsberg erecta ecotype, gene CESA7
-

Purification (Commentary)

Purification (Comment) Organism
native oligomers partially from microsomes as fragments of the cellulose synthase complex by dual epitope tagging of the CESA7 protein, selection for CESA multimers, overview. Recombinant His- and STREP-tagged CESA4, CESA7, and CESA8, as well as double mutant irx3-1:STREP-CESA7-irx3-1:His/FLAG-CESA7 from Arabidopsis thaliana irx1-1, irx3-1, and irx5-2 mutant plants by nickel and streptavidin affinity chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
stem
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information cellulose synthase A is a catalytic component of the cellulose synthase complex Arabidopsis thaliana ?
-
 ?

Subunits

Subunits Comment Organism
More cellulose synthase A is a catalytic component of the cellulose synthase complex. Mass spectrometric analysis of purified cellulose synthase components, His- and STREP-tagged CESA4, CESA7, and CESA8 from irx1-1, irx3-1, and irx5-2 mutant plants, overview Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
cellulose synthase A
-
 Arabidopsis thaliana
cellulose synthase complex
-
 Arabidopsis thaliana
CesA
-
 Arabidopsis thaliana
CSC
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction the naturally occuring irx3-1 and irx5-2 mutations are caused by premature stop codons that result in protein truncation of CESA7 and CESA4,respectively. In the naturally occuring irx3-1 background, interaction between CESA4 and CESA8 is greatly reduced, and the proteins fail to localize to the plasma membrane Arabidopsis thaliana