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Literature summary for 2.4.1.117 extracted from

  • Villemez, C.L.; Carlo, P.L.
    Properties of a soluble polyprenyl phosphate: UDP-D-glucose glucosyltransferase (1979), J. Biol. Chem., 254, 4814-4819.
    View publication on PubMed
Search for more literature for 2.4.1.117

Activating Compound

Activating Compound Comment Organism Structure
Reducing agent required Acanthamoeba castellanii

Inhibitors

Inhibitors Comment Organism Structure
EDTA inhibition reversed by MgCl2 Acanthamoeba castellanii
UDP competitive Acanthamoeba castellanii
UMP reversible Acanthamoeba castellanii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0045
-
 dolichyl phosphate
-
 Acanthamoeba castellanii
0.0091
-
 UDP-glucose
-
 Acanthamoeba castellanii

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Acanthamoeba castellanii
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ divalent cation required, either Mg2+ or Mn2+, Mn2+ stimulates the enzyme to a greater extent than Mg2+ at low concentrations, Mg2+ stimulates to a greater extent than Mn2+ at high concentration, but each is maximally effective at a concentration around 8 mM Acanthamoeba castellanii
Mn2+ divalent cation required, either Mg2+ or Mn2+, Mn2+ stimulates the enzyme to a greater extent than Mg2+ at low concentrations, Mg2+ stimulates to a greater extent than Mn2+ at high concentration, but each is maximally effective at a concentration around 8 mM Acanthamoeba castellanii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Acanthamoeba castellanii synthesis of dolichyl D-glucosyl phosphate, a glucosyl donor in the formation of lipid-linked core oligosaccharides ?
-
 ?
UDP-glucose + dolichyl phosphate Acanthamoeba castellanii
-
 UDP + dolichyl beta-D-glucosyl phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Acanthamoeba castellanii
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cyst
-
 Acanthamoeba castellanii
-

Storage Stability

Storage Stability Organism
-20°C, indefinitely stable below Acanthamoeba castellanii
4°C, stable for several days Acanthamoeba castellanii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no substrate: GDP-D-glucose, UDP-D-glucuronic acid, UDP-N-acetyl-D-glucosamine, UDP-D-xylose Acanthamoeba castellanii ?
-
 ?
additional information synthesis of dolichyl D-glucosyl phosphate, a glucosyl donor in the formation of lipid-linked core oligosaccharides Acanthamoeba castellanii ?
-
 ?
UDP-glucose + dolichyl phosphate
-
 Acanthamoeba castellanii UDP + dolichyl beta-D-glucosyl phosphate
-
 r
UDP-glucose + ficaprenyl phosphate
-
 Acanthamoeba castellanii UDP + ficaprenyl beta-D-glucosyl phosphate
-
 r
UDP-glucose + solanesyl phosphate
-
 Acanthamoeba castellanii UDP + solanesyl beta-D-glucosyl phosphate
-
 r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Acanthamoeba castellanii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 2 h, 50% loss of activity Acanthamoeba castellanii
52
-
 10 min, complete loss of activity Acanthamoeba castellanii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Acanthamoeba castellanii

pH Range

pH Minimum pH Maximum Comment Organism
5.6 8.8 pH 5.6: 25% of maximum activity, pH 8.8: about 40% of maximum activity Acanthamoeba castellanii