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Literature summary for 2.4.1.116 extracted from
- Identification, properties, and genetic control of UDP-glucose: cyanidin-3-rhamnosyl-(1 -6)-glucoside-5-O-glucosyltransferase isolated from petals of the red campion (Silene dioica) (1978), Biochem. Genet., 16, 1059-1071.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, highest stimulation of divalent cations | Silene dioica |  |
| Co2+ | 1 mM activates | Silene dioica | |
| Mg2+ | 1 mM activates | Silene dioica | |
| Mn2+ | 1 mM activates | Silene dioica | |
| additional information | influence of detergents and reducing agents on enzyme activity in crude extracts | Silene dioica | |
| Polyvinylpyrrolidone | soluble, required for maximal activity in crude extracts | Silene dioica | |
General Stability
| General Stability | Organism |
|---|---|
| repeated freeze-thawing inactivates | Silene dioica |
| repeated freezing and thawing leads to a rapid decline in activity | Silene dioica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 1 mM, 57% inhibition | Silene dioica | |
| Hg2+ | - |
Silene dioica | |
| HgCl2 | 0.1 mM, 95% inhibition, irreversible inhibition | Silene dioica | |
| N-ethylmaleimide | 3 mM, 25% inhibition | Silene dioica | |
| NEM | 3 mM, 25% inhibition, 2-mercpatoethanol or cysteine does not protect | Silene dioica | |
| p-chloromercuribenzoate | 3 mM, 25% inhibition | Silene dioica | |
| PCMB | 3 mM, 25% inhibition, 2-mercpatoethanol or cysteine does not protect | Silene dioica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
UDPglucose | reaction with cyanidin 3-rhamnosylglucoside at pH 7.4 | Silene dioica | |
| 0.5 | - |
UDP-glucose | pH 7.4, 30°C | Silene dioica | |
| 3.6 | - |
cyanidin-3-O-D-rhamnosyl-1,6-D-glucoside | - |
Silene dioica | |
| 3.6 | - |
cyanidin-3-O-rutinoside | pH 7.4, 30°C | Silene dioica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | 1 mM, 180% stimulation, highest stimulation | Silene dioica | |
| Co2+ | 1 mM, stimulation | Silene dioica | |
| Mg2+ | 1 mM, stimulation | Silene dioica | |
| Mn2+ | 1 mM, stimulation | Silene dioica | |
| additional information | enzyme is stimulated by divalent metal ions | Silene dioica |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
gel filtration | Silene dioica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + cyanidin 3-O-beta-D-rutinoside | Silene dioica | biosynthetic pathway of cyanidin-3-rhamnosylglucoside-5-glucoside, enzyme activity is controlled by a single dominant gene M | UDP + cyanidin 3-O-beta-D-rutinoside 5-O-beta-D-glucoside | - |
? | |
| UDPglucose + cyanidin-3-O-D-rhamnosyl-1,6-D-glucoside | Silene dioica | involved in anthocyan biosynthesis. Activity is controlled by a single dominant gene | UDP + cyanidin-3-O-[D-rhamnosyl-1,6-D-glucoside]-5-O-D-glucoside | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Silene dioica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 79.4fold | Silene dioica |
| partial | Silene dioica |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| UDP-alpha-D-glucose + cyanidin-3-O-rutinoside = UDP + cyanidin 3-O-rutinoside 5-O-beta-D-glucoside | also acts on pelargonidin-3-rutinoside, the enzyme does not catalyse the glucosylation of the 5-hydroxy group of cyanidin-3-glucoside | Silene dioica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | not in green parts of the plant | Silene dioica | - |
| petal | - |
Silene dioica | - |
| petal | not in green parts of the plant | Silene dioica | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00953 | - |
- |
Silene dioica |
| 0.00953 | - |
pH 7.4, 30°C | Silene dioica |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, about 60-80% loss of activity within 3 months | Silene dioica |
| -20°C, concentrated enzyme preparation, 3 months, 60-80% loss of activity | Silene dioica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | not: ADP-glucose, enzyme can use different substrates for 5-O-glycosylation depending on the pH | Silene dioica | ? | - |
? | |
| UDP-alpha-D-glucose + cyanidin 3-O-beta-D-rutinoside | biosynthetic pathway of cyanidin-3-rhamnosylglucoside-5-glucoside, enzyme activity is controlled by a single dominant gene M | Silene dioica | UDP + cyanidin 3-O-beta-D-rutinoside 5-O-beta-D-glucoside | - |
? | |
| UDP-alpha-D-glucose + cyanidin 3-O-beta-D-rutinoside | catalyzes the transfer of the glucosyl moiety of UDP-glucose to the 5-hydroxyl group of cyanidin-3-rhamnosyl-(1?6)-glucoside | Silene dioica | UDP + cyanidin 3-O-beta-D-rutinoside 5-O-beta-D-glucoside | - |
? | |
| UDP-alpha-D-glucose + pelargonidin 3-O-(6-O-alpha-L-rhamnosyl)-beta-D-glucoside | - |
Silene dioica | UDP + pelargonidin 3-O-(6-O-alpha-L-rhamnosyl)-beta-D-glucosyl-5-O-beta-D-glucoside | - |
? | |
| UDP-glucose + cyanidin-3-O-D-rhamnosyl-1,6-D-glucoside | - |
Silene dioica | UDP + cyanidin-3-O-[D-rhamnosyl-1,6-D-glucoside]-5-O-D-glucoside | - |
r | |
| UDP-glucose + cyanidin-3-O-glucoside | at a much lower rate than cyanidin-3-O-rutinoside, at pH 6.5, but not at pH 7.4, enzyme can use different substrates for 5-O-glycosylation depending on the pH | Silene dioica | UDP + cyanidin-3,5-diglucoside | - |
? | |
| UDP-glucose + pelargonidin-3-rutinoside | also uses pelargonidin-3-rhamnosylglucoside, glucosylates the 5-hydroxyl group | Silene dioica | UDP + pelargonidin-3-rutinoside-5-O-beta-D-glucoside | - |
? | |
| UDPglucose + cyanidin-3-O-D-rhamnosyl-1,6-D-glucoside | no activity with ADPglucose | Silene dioica | UDP + cyanidin-3-O-[D-rhamnosyl-1,6-D-glucoside]-5-O-D-glucoside | - |
? | |
| UDPglucose + cyanidin-3-O-D-rhamnosyl-1,6-D-glucoside | involved in anthocyan biosynthesis. Activity is controlled by a single dominant gene | Silene dioica | UDP + cyanidin-3-O-[D-rhamnosyl-1,6-D-glucoside]-5-O-D-glucoside | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UDP-glucose:cyanidin-3-rhamnosyl-(1-6)-glucoside-5-O-glucosyltransferase | - |
Silene dioica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Silene dioica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
glucosyl transfer to cyanidin-3-O-glucoside, substrate specificity is pH-dependent | Silene dioica |
| 7.4 | - |
- |
Silene dioica |
| 7.4 | - |
glucosyl transfer to cyanidin-3-O-rutinoside and pelargonidin-3-rutinoside, substrate specificity is pH-dependent | Silene dioica |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.9 | 8.8 | about 50% of maximal activity at pH 6.9 and at pH 8.8 | Silene dioica |
| 6.9 | 8.8 | half-maximum velocities at pH 6.9 and 8.8, cyanidin-3-O-rutinoside as substrate | Silene dioica |
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