KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0108 | - |
UDPglucose | pH 7.4, 30°C | Kitasatospora aureofaciens | |
0.11 | - |
alizarin | pH 7.4, 30°C | Kitasatospora aureofaciens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Kitasatospora aureofaciens | 5737 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kitasatospora aureofaciens | - |
mutant strain B96 | - |
Purification (Comment) | Organism |
---|---|
partial | Kitasatospora aureofaciens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-glucose + 1,2-dihydroxy-9,10-anthraquinone = UDP + 1-hydroxy-2-(beta-D-glucosyloxy)-9,10-anthraquinone | mechanism | Kitasatospora aureofaciens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Kitasatospora aureofaciens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Kitasatospora aureofaciens |
Storage Stability | Organism |
---|---|
-20°C, 2 months | Kitasatospora aureofaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + 1,4-dihydroxy-9,10-anthraquinone | 7% of the activity with 1,2-dihydroxy-9,10-anthraquinone | Kitasatospora aureofaciens | UDP + 1-hydroxy-4-(beta-D-glucosyloxy)-9,10-anthraquinone | - |
? | |
UDP-glucose + 1,5-dihydroxy-9,10-anthraquinone | 2% of the activity with 1,2-dihydroxy-9,10-anthraquinone | Kitasatospora aureofaciens | UDP + 1-hydroxy-5-(beta-D-glucosyloxy)-9,10-anthraquinone | - |
? | |
UDP-glucose + alizarin | i.e.1,2-dihydroxy-9,10-anthraquinone | Kitasatospora aureofaciens | UDP + 1-hydroxy-2-(beta-D-glucosyloxy)-9,10-anthraquinone | - |
? | |
UDPglucose + 1,3-dihydroxy-9,10-anthraquinone | 48% of the activity with 1,2-dihydroxy-9,10-anthraquinone | Kitasatospora aureofaciens | UDP + 1-hydroxy-3-(beta-D-glucosyloxy)-9,10-anthraquinone | - |
? | |
UDPglucose + 1,8-dihydroxy-9,10-anthraquinone | 9% of the activity with 1,2-dihydroxy-9,10-anthraquinone | Kitasatospora aureofaciens | UDP + 1-hydroxy-8-(beta-D-glucosyloxy)-9,10-anthraquinone | - |
? | |
UDPglucose + 1-hydroxy-3-methoxy9,10-anthraquinone | 2% of the activity with 1,2-dihydroxy-9,10-anthraquinone | Kitasatospora aureofaciens | UDP + 3-methoxy-1-(beta-D-glucosyloxy)-9,10-anthraquinone | - |
? | |
UDPglucose + 1-hydroxy-9,10-anthraquinone | 3% of the activity with 1,2-dihydroxy-9,10-anthraquinone | Kitasatospora aureofaciens | UDP + 1-(beta-D-glucosyloxy)-9,10-anthraquinone | - |
? | |
UDPglucose + 2,6-dihydroxy-9,10-anthraquinone | 51% of the activity with 1,2-dihydroxy-9,10-anthraquinone | Kitasatospora aureofaciens | UDP + 1-hydroxy-6-(beta-D-glucosyloxy)-9,10-anthraquinone | - |
? | |
UDPglucose + 2-hydroxy-9,10-anthraquinone | 32% of the activity with 1,2-dihydroxy-9,10-anthraquinone | Kitasatospora aureofaciens | UDP + 2-(beta-D-glucosyloxy)-9,10-anthraquinone | - |
? | |
UDPglucose + 3-hydroxy-1-methoxy-9,10-anthraquinone | 52% of the activity with 1,2-dihydroxy-9,10-anthraquinone | Kitasatospora aureofaciens | UDP + 1-methoxy-3-(beta-D-glucosyloxy)-9,10-anthraquinone | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Kitasatospora aureofaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
glucosylation of 1,2-dihydroxy-9,10-anthraquinone | Kitasatospora aureofaciens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.8 | 7.6 | about half-maximal activity at pH 6.7 and 7.6 | Kitasatospora aureofaciens |