Literature summary for 2.4.1.102 extracted from

Lete, C.; Markine-Goriaynoff, N.; Machiels, B.; Pang, P.; Xiao, X.; Canis, K.; Suzuki, M.; Fukuda, M.; Dell, A.; Haslam, S.; Vanderplasschen, A.; Gillet, L.
Bovine herpesvirus 4 modulates its beta-1,6-n-acetylglucosaminyltransferase activity through alternative splicing (2016), J. Virol., 90, 2039-2051 .

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Cloned(Commentary)

Cloned (Comment)	Organism
the Bo17 gene of Bovine herpesvirus 4 (BoHV-4) encodes a homologue of the cellular core 2 protein beta-1,6-N-acetylglucosaminyltransferase-mucin type (C2GnT-M), which is a key player for the synthesis of complex O-glycans. As opposed to what is observed for the cellular enzyme, two different mRNAs are encoded by the Bo17 gene of all available BoHV-4 strains. Bo17 undergoes an alternative splicing that generates two different products of expression. While the first one corresponds to the entire coding sequence of the Bo17 gene, the second results from the splicing of a 138-bp intron encoding critical residues of the enzyme. The two forms of Bo17 are expressed in BoHV-4 infected cells, but the spliced form is not active, recombinant expression of only the long or the short form of Bo17, RT-PCR expression analysis	Bovine gammaherpesvirus 4

Cloned (Comment)

Organism

the Bo17 gene of Bovine herpesvirus 4 (BoHV-4) encodes a homologue of the cellular core 2 protein beta-1,6-N-acetylglucosaminyltransferase-mucin type (C2GnT-M), which is a key player for the synthesis of complex O-glycans. As opposed to what is observed for the cellular enzyme, two different mRNAs are encoded by the Bo17 gene of all available BoHV-4 strains. Bo17 undergoes an alternative splicing that generates two different products of expression. While the first one corresponds to the entire coding sequence of the Bo17 gene, the second results from the splicing of a 138-bp intron encoding critical residues of the enzyme. The two forms of Bo17 are expressed in BoHV-4 infected cells, but the spliced form is not active, recombinant expression of only the long or the short form of Bo17, RT-PCR expression analysis

Bovine gammaherpesvirus 4

Protein Variants

Protein Variants	Comment	Organism
additional information	production of BoHV-4 Bo17 mutant strains, i.e. the BoHV-4 V.test strain deleted for the Bo17 ORF (Bo17 Del) and the corresponding revertant strain (Bo17 Rev). The recombinant strains Bo17 MuDir and Bo17 Spliced are derived from a cloned BoHV-4 bacterial artificial chromosome (BAC). The Bo17 MuDir sequence is generated by site-directed mutagenesis, two mutations are inserted in the 5' splice site and one mutation in the 3' splice site. O-Glycomic profile analysis of wild-type and mutant strains. The Bo17 knockout strain does not display any growth deficit in vitro. Analysis of the structures of O-glycans isolated from cells mock infected or infected with the Bo17 Del, Bo17 MuDir, Bo17 MuDir Rev, Bo17 Spliced, and Bo17 Spliced Rev strains of BoHV-4. The infection by wild-type, Bo17 MuDir, and the revertant strains is moderately inhibited by jacalin, this neutralization is significantly stronger for the Bo17 Del strain and even more for the Bo17 Spliced strain	Bovine gammaherpesvirus 4

Organism

Organism	UniProt	Comment	Textmining
Bovine gammaherpesvirus 4	-	BoHV-4, strains LVR 140, MOVAR 33/63, DN599, 66-P-347, 108, 130, and Buf representative of the BoHV-4 species isolated throughout the world and from different animal species	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	production of Anti-Bo17 422-441 polyclonal monospecific antibodies from BoHV-4 V.test strain Bo17 protein and immunohistochemic analysis	Bovine gammaherpesvirus 4	-

Synonyms

Synonyms	Comment	Organism
beta-1,6-N-acetylglucosaminyltransferase	-	Bovine gammaherpesvirus 4
Bo17	-	Bovine gammaherpesvirus 4
c2GnT-M	-	Bovine gammaherpesvirus 4
Core2beta1,6GnT type M	-	Bovine gammaherpesvirus 4

General Information

General Information	Comment	Organism
evolution	the viral 17 gene encodes a functional homologue of the cellular Corebeta1,6GnT typeM(C2GnT-M), which was acquired from a recent ancestor of the African buffalo. Bo17 splicing is conserved among BoHV-4 strains but not in its cellular counterparts	Bovine gammaherpesvirus 4
malfunction	the Bo17 knockout strain does not display any growth deficit in vitro	Bovine gammaherpesvirus 4
additional information	the splicing of the Bo17 gene gives the potential to BoHV-4 to fine-tune the global level of core 2 branching activity in the infected cell. Analysis of the structures of O-glycans isolated from cells mock infected or infected with the Bo17 Del, Bo17 MuDir, Bo17 MuDir Rev, Bo17 Spliced, and Bo17 Spliced Rev strains of BoHV-4	Bovine gammaherpesvirus 4
physiological function	the Bo17 gene is nonessential for virus replication, despite its contributing to posttranslational modification of virion proteins. Bo17 undergoes an alternative splicing that generates two different products of expression. In contrast to the full-length form of the protein, the shortened spliced form is devoid of enzymatic activity and confers to BoHV-4 the potential to fine-tune the core 2 branching activity of the infected cell, mechanisms to regulate the activity of glycosyltransferases from the Golgi apparatus, overview. O-Glycomic profile analysis of wild-type and mutant strains. Sole expression of either of the two Bo17-encoded proteins can influence BoHV-4 growth. Bo17 expression and splicing may affect the structure of O-glycans on BoHV-4 virions and in particular on glycoprotein gp180	Bovine gammaherpesvirus 4