Cloned (Comment) | Organism |
---|---|
gene Bo17, DNA and amino acid sequence determination and analysis, two different mRNAs are encoded by the Bo17 gene of all available BoHV-4 strains. While the first one corresponds to the entire coding sequence of the Bo17 gene, the second results from the splicing of a 138-bp intron encoding critical residues of the enzyme. The first one is enzymatically active, the second is inactive and results from the alternative splicing of the region encoding the catalytic site of the enzyme, enzyme activities in infected cells, overview | Bovine gammaherpesvirus 4 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
jacalin | core 1 O-glycan-specific lectin from Artocarpus integrifolia, differing sensitivity of different BoHV-4 mutants to jacalin-mediated neutralization, overview | Bovine gammaherpesvirus 4 | |
additional information | Bo17 splicing reduces BoHV-4 glycosylation | Bovine gammaherpesvirus 4 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bovine gammaherpesvirus 4 | Q9IZK2 | BoHV-4, gene Bo17 | - |
Bovine gammaherpesvirus 4 V. test | Q9IZK2 | BoHV-4, gene Bo17 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | infection of MDBK cells with strain BoHV-4 V.test | Bovine gammaherpesvirus 4 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + glycoprotein gp180 | - |
Bovine gammaherpesvirus 4 | UDP + N-acetyl-beta-D-glucosaminyl-glycoprotein gp180 | - |
? | |
UDP-N-acetyl-D-glucosamine + glycoprotein gp180 | - |
Bovine gammaherpesvirus 4 V. test | UDP + N-acetyl-beta-D-glucosaminyl-glycoprotein gp180 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-1,6-N-acetylglucosaminyltransferase | - |
Bovine gammaherpesvirus 4 |
c2GnT-M | - |
Bovine gammaherpesvirus 4 |
core 2 protein beta-1,6-N-acetylglucosaminyltransferase-mucin type | - |
Bovine gammaherpesvirus 4 |
General Information | Comment | Organism |
---|---|---|
evolution | in contrast to cellular homologues, this virus has evolved to alternatively express two proteins from gene Bo17 | Bovine gammaherpesvirus 4 |
malfunction | a Bo17 knockout strain does not display any growth deficit in vitro. But sole expression of either of the two Bo17-encoded proteins could influence BoHV-4 growth | Bovine gammaherpesvirus 4 |
additional information | the two isoforms of Bo17 are expressed in BoHV-4 infected cells, but enzymatic assays revealed that the spliced form is not active | Bovine gammaherpesvirus 4 |
physiological function | Bovine herpesvirus 4 modulates its beta-1,6-N-acetylglucosaminyltransferase activity through alternative splicing. The enzyme is a homologue of the cellular core 2 protein beta-1,6-N-acetylglucosaminyltransferase-mucin type (C2GnT-M), which is a key player for the synthesis of complex O-glycans | Bovine gammaherpesvirus 4 |