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Literature summary for 2.4.1.102 extracted from

  • Petrosyan, A.; Ali, M.F.; Cheng, P.W.
    Keratin 1 plays a critical role in Golgi localization of core 2 N-acetylglucosaminyltransferase M via interaction with its cytoplasmic tail (2015), J. Biol. Chem., 290, 6256-6269.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.102

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of C-terminally c-Myc-tagged betaC2GnT-M in Panc1 cells, transient expression of GFP-tagged C2GnT-M in HeLa cells. Yeast two-hybrid analysis of the interactions between KRT1 protein head, tail, or rod domain and the cytoplasmic peptides of hC2GnT-M Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus Keratin 1 retains C2GnT-M in the Golgi by interacting with its cytoplasmic tail via the rod domain Homo sapiens 5794
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additional information isozyme C2GnT-M targets the Golgi at the Giantin site and is recycled by binding to non-muscle myosin IIA, a motor protein, via the cytoplasmic tail. C2GnT-M forms a complex with Giantin before KRT1, demonstrating CT-mediated sequential events of Golgi targeting and retention of C2GnT-M Homo sapiens
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Organism

Organism UniProt Comment Textmining
Homo sapiens O95395
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Source Tissue

Source Tissue Comment Organism Textmining
colon epithelial cell
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 Homo sapiens
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PANC-1 cell
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 Homo sapiens
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information KRT1 binds the biotinylated CT peptide MVQWKRL of C2GnT-M isozyme Homo sapiens ?
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 ?

Synonyms

Synonyms Comment Organism
c2GnT-M
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 Homo sapiens
Core 2 N-acetylglucosaminyltransferase 2/M
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 Homo sapiens
core 2 N-acetylglucosaminyltransferase M
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 Homo sapiens

General Information

General Information Comment Organism
malfunction loss of C2GnT-M leads to development of colitis and colon cancer. Knockdown of KRT1 by siRNA does not affect Golgi morphology but leaves C2GnT-M outside of the Golgi, resulting in the formation of sialyl-T antigen Homo sapiens
additional information Keratin 1 retains C2GnT-M in the Golgi by interacting with its cytoplasmic tail via the rod domain. Keratin 1 plays a critical role in the regulation of O-glycosylation pathways Homo sapiens
physiological function mucin-type isozyme core 2 N-acetylglucosaminyltransferase 2/M synthesizes all three beta6GlcNAc branch structures found in secreted mucins Homo sapiens