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Literature summary for 2.4.1.102 extracted from

  • Yen, T.Y.; Macher, B.A.; Bryson, S.; Chang, X.; Tvaroska, I.; Tse, R.; Takeshita, S.; Lew, A.M.; Datti, A.
    Highly conserved cysteines of mouse core 2 beta1,6-N-acetylglucosaminyltransferase I form a network of disulfide bonds and include a thiol that affects enzyme activity (2003), J. Biol. Chem., 278, 45864-45881.
    View publication on PubMed
Search for more literature for 2.4.1.102

Protein Variants

Protein Variants Comment Organism
C217S Cys217 although not required for activity per se, represents the only thiol that causes enzyme inactivation when modified. KM-value for UDP-N-acetyl-D-glucosamine is about 5% higher than the wild-type value, the Km-value for Galbeta(1-3)GalNAcalpha-p-nitrophenol is about 20% higher than the wild-type value.Unlike the wild-type enzyme the mutant enzyme can be stored at 4°C for at least 3 months without any loss of activity. The mutant enzyme is insensitive to DTNB, iodoacetamide, NEM, HgCl2 and ZnCl2 Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
additional information the mutant enzyme C217S is insensitive to DTNB, iodoacetamide, NEM, HgCl2 and ZnCl2 Mus musculus
UDP competitive inhibition versus donor substrate, wild-type enzyme and mutant enzyme C217S Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 Galbeta(1-3)GalNAcalpha-p-nitrophenol pH 6.7, 37°C, wild-type enzyme Mus musculus
0.13
-
 Galbeta(1-3)GalNAcalpha-p-nitrophenol pH 6.7, 37°C, mutant enzyme C217S Mus musculus
1.1
-
 UDP-N-acetyl-D-glucosamine pH 6.7, 37°C, wild-type enzyme Mus musculus
1.15
-
 UDP-N-acetyl-D-glucosamine pH 6.7, 37°C, mutant enzyme C217S Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Oxidation Stability

Oxidation Stability Organism
inactivation during storage may be caused by air oxidation and points to a requirement for inmodified thiols in the core protein Mus musculus

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein both Asn58 and Asn95 are glycosylated Mus musculus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.2
-
 mutant enzyme C217S Mus musculus
9.2
-
 wild-type enzyme Mus musculus

Storage Stability

Storage Stability Organism
mutant enzyme C217S can be stored at 4°C for at least 3 months without any loss of activity, wild-type enzyme loses about 95% of its activity after 4 weeks Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-glucosamine + Galbeta(1-3)GalNAcalpha-p-nitrophenol
-
 Mus musculus UDP + ?
-
 ?

Subunits

Subunits Comment Organism
More in absence of dithiothreitol, one of the two free Cys residues (Cys217 and Cys235) is found to be engaged in an intermolecular bridge causing dimerization of the protein Mus musculus

Synonyms

Synonyms Comment Organism
core 2 beta1,6-N-acetylglucosaminyltransferase I
-
 Mus musculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.14
-
 UDP wild-type enzyme Mus musculus
0.15
-
 UDP mutant enzyme C217S Mus musculus