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Literature summary for 2.4.1.102 extracted from
- Mucin synthesis. I. Detection in canine submaxillary glands of an N-acetylglucosaminyltransferase which acts on mucin substrates (1980), J. Biol. Chem., 255, 11247-11252.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Empigen BB | activation, detergent | Canis lupus familiaris |  |
| Triton X-100 | 4fold stimulation at 0.125% v/v | Canis lupus familiaris | |
General Stability
| General Stability | Organism |
|---|---|
| freeze-thawing, after 2 cycles 13% loss of activity | Canis lupus familiaris |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | slight inhibition | Canis lupus familiaris | |
| EDTA | slight inhibition | Canis lupus familiaris | |
| Mn2+ | slight stimulation at 5 mM, inhibition at higher concentrations | Canis lupus familiaris | |
| Zn2+ | strong inhibition | Canis lupus familiaris | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
UDP-N-acetyl-D-glucosamine | - |
Canis lupus familiaris | |
| 5.2 | - |
asialo-blood group A negative PSM | - |
Canis lupus familiaris |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Canis lupus familiaris | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | slight stimulation, 5 mM | Canis lupus familiaris | |
| Mn2+ | slight stimulation, 5 mM, inhibits at higher concentration | Canis lupus familiaris | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Canis lupus familiaris | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| submaxillary gland | - |
Canis lupus familiaris | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| frozen in liquid nitrogen, several months | Canis lupus familiaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-D-glucosamine + asialo-alpha1 acid glycoprotein | - |
Canis lupus familiaris | ? | - |
? | |
| UDP-N-acetyl-D-glucosamine + asialo-blood group A negative PSM | acceptor contains no fucose | Canis lupus familiaris | ? | - |
? | |
| UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-benzyl | - |
Canis lupus familiaris | UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-benzyl | - |
? | |
| UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-phenyl | - |
Canis lupus familiaris | UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-phenyl | - |
? | |
| UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R | - |
Canis lupus familiaris | UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R | i.e. core class 2 | ? | |
| UDP-N-acetyl-D-glucosamine + submaxillary mucin polypeptide | porcine or ovine origin of acceptor substrate | Canis lupus familiaris | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Canis lupus familiaris |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
25°C, 2 h | Canis lupus familiaris |
| 37 | - |
unstable | Canis lupus familiaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Canis lupus familiaris |
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