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Literature summary for 2.4.1.101 extracted from

  • Damerow, M.; Rodrigues, J.A.; Wu, D.; Guether, M.L.; Mehlert, A.; Ferguson, M.A.
    Identification and functional characterization of a highly divergent N-acetylglucosaminyltransferase I (TbGnTI) in Trypanosoma brucei (2014), J. Biol. Chem., 289, 9328-9339.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene gene TbGT11, DNA and amino acid sequence determination and analysis, gene TbGT11 encodes a Golgi apparatus resident UDP-GlcNAc:alpha3-D-mannoside beta1-2-N-acetylglucosaminyltransferaseI activity (TbGnTI).overexpression of full-length TbGT11 with a C-terminal HA3 epitope tag from a plasmid based on the trypanosome expression vector pLEW82 Trypanosoma brucei brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus
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Trypanosoma brucei brucei 5794
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-D-glucosamine + 3-(alpha-D-mannosyl)-beta-D-mannosyl-R Trypanosoma brucei brucei
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UDP + 3-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
-
?
UDP-N-acetyl-D-glucosamine + 3-(alpha-D-mannosyl)-beta-D-mannosyl-R Trypanosoma brucei brucei 427
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UDP + 3-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
-
?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei brucei
-
expressing VSG variant 221 and transformed to stably express T7 polymerase and the tetracycline repressor protein under G418 antibiotic selection, gene TbGT11 or Tb427.3.5660
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Trypanosoma brucei brucei 427
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expressing VSG variant 221 and transformed to stably express T7 polymerase and the tetracycline repressor protein under G418 antibiotic selection, gene TbGT11 or Tb427.3.5660
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-glucosamine + 3-(alpha-D-mannosyl)-beta-D-mannosyl-R
-
Trypanosoma brucei brucei UDP + 3-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
-
?
UDP-N-acetyl-D-glucosamine + 3-(alpha-D-mannosyl)-beta-D-mannosyl-R enzyme TbGnTI transfers UDP-GlcNAc to biantennary Man3GlcNAc2, but not to triantennary Man5GlcNAc2, which is the preferred substrate for metazoan enzymes Trypanosoma brucei brucei UDP + 3-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
-
?
UDP-N-acetyl-D-glucosamine + 3-(alpha-D-mannosyl)-beta-D-mannosyl-R
-
Trypanosoma brucei brucei 427 UDP + 3-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
-
?
UDP-N-acetyl-D-glucosamine + 3-(alpha-D-mannosyl)-beta-D-mannosyl-R enzyme TbGnTI transfers UDP-GlcNAc to biantennary Man3GlcNAc2, but not to triantennary Man5GlcNAc2, which is the preferred substrate for metazoan enzymes Trypanosoma brucei brucei 427 UDP + 3-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
-
?

Synonyms

Synonyms Comment Organism
GnTI
-
Trypanosoma brucei brucei
TbGnTI
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Trypanosoma brucei brucei
UDP-GlcNAc:alpha3-D-mannoside beta1-2-N-acetylglucosaminyltransferase I
-
Trypanosoma brucei brucei

General Information

General Information Comment Organism
evolution sequence alignment reveals that the Trypanosoma brucei enzyme is far removed from the metazoan GnTI family and suggests that the parasite has adapted the beta3-glycosyltransferase family to catalyze beta1-2 linkages. It belongs to a family of putative UDP-sugar-dependent glycosyltransferases with similarity to the mammalian beta1-3-glycosyltransferase family. Enzyme TbGnTI transfers UDP-GlcNAc to biantennary Man3GlcNAc2, but not to triantennary Man5GlcNAc2, which is the preferred substrate for metazoan enzymes Trypanosoma brucei brucei
malfunction the bloodstream-form TbGT11 null mutant exhibits significantly modified protein N-glycans but normal growth in vitro and infectivity to rodents Trypanosoma brucei brucei
metabolism the first step in hybrid and complex N-glycan biosynthesis is initiated by N-acetylglucosaminyltransferase I (GnTI) through the addition of an N-acetylglucosamine (GlcNAc) residue to the alpha1-3-linked core mannose of Man5GlcNAc2 Trypanosoma brucei brucei
physiological function in contrast to multicellular organisms, where the enzyme activity is essential for biosynthesis of both complex and hybrid N-glycans, Trypanosoma brucei TbGT11 null mutants produce atypical pseudohybrid glycans, indicating that the enzyme activity is not dependent on prior TbGnTI action. The enzyme GnTI reaction is essential for biosynthesis of both complex and hybridN-glycans. TbGnTII activity is not dependent on prior TbGnTI action Trypanosoma brucei brucei