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Literature summary for 2.4.1.101 extracted from

  • Chen, W.; Unligil, U.M.; Rini, J.M.; Stanley, P.
    Independent Lec1A CHO glycosylation mutants arise from point mutations in N-acetylglucosaminyltransferase I that reduce affinity for both substrates. Molecular consequences based on the crystal structure of GlcNAc-TI (2001), Biochemistry, 40, 8765-8772.
    View publication on PubMed
Search for more literature for 2.4.1.101

Protein Variants

Protein Variants Comment Organism
C907T point mutation in mutant cell line Lec1A.3E and Lec1A.5J, altering interactions important for stabilization of the structure, correction of the mutation by site-directed mutagenesis restores enzyme activity Cricetulus griseus
G634A point mutation in mutant cell line Lec1A.2C, disrution of DxD motif responsible for interaction with UDP-GlcNAc and Mn2+, correction of the mutation by site-directed mutagenesis restores enzyme activity Cricetulus griseus
additional information 1 difference to published sequence with accession number U65791: C1340 is A1340 Cricetulus griseus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
 UDP-N-acetyl-D-glucosamine wild-type CHO Cricetulus griseus
0.26
-
 Man5GlcNAc2 wild-type CHO Cricetulus griseus
5.43
-
 Man5GlcNAc2 mutant Lec1A Cricetulus griseus
7.56
-
 UDP-N-acetyl-D-glucosamine mutant Lec1A Cricetulus griseus

Organism

Organism UniProt Comment Textmining
Cricetulus griseus P70680 1 difference to published sequence U65791: C1340 is A1340
-
Mesocricetus auratus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
UDP-N-acetyl-alpha-D-glucosamine + Man5GlcNAc2-[protein] = UDP + Man5GlcNAc3-[protein] active site Cricetulus griseus
a
UDP-N-acetyl-alpha-D-glucosamine + Man5GlcNAc2-[protein] = UDP + Man5GlcNAc3-[protein] R303 is important for enzyme activity, conserved throughout all species Cricetulus griseus
a
UDP-N-acetyl-alpha-D-glucosamine + Man5GlcNAc2-[protein] = UDP + Man5GlcNAc3-[protein] D212 is the central residue of the DxD motif, conserved throughout all species Cricetulus griseus
a

Source Tissue

Source Tissue Comment Organism Textmining
CHO cell wild-type, Lec1 cells are deficient in enzyme activity, mutant Lec1A cells show weak enzyme activity, 2% compared to wild-type, and produce complex N-glycans Cricetulus griseus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 wild-type and mutant cell lines Cricetulus griseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-glucosamine + Man5GlcNAc2
-
 Cricetulus griseus ?
-
 ?

Synonyms

Synonyms Comment Organism
GlcNAc-T I
-
 Mesocricetus auratus
GlcNAc-T I
-
 Cricetulus griseus