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Literature summary for 2.4.1.101 extracted from

  • Mendicino, J.; Chandrasekaran, E.V.; Rao Anumula, K.; Davila, M.
    Isolation and properties of alpha-D-mannose: beta-1,2-N-acetylglucosaminyltransferase from trachea mucosa (1981), Biochemistry, 20, 967-976.
    View publication on PubMed
Search for more literature for 2.4.1.101

General Stability

General Stability Organism
freeze-thawing, solubilized enzyme, unstable to Sus scrofa
Triton X-100, 0.1%, and albumin, 0.01%, stabilize solubilized enzyme Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Sus scrofa 5829
-
microsome
-
 Sus scrofa
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ requirement Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
tracheal mucosa
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.37
-
 purified enzyme Sus scrofa

Storage Stability

Storage Stability Organism
3°C, at least a month Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R substrate specificity Sus scrofa UDP + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R transfers an N-acetylglucosamine in beta-1,2-linkage to mannosyl-1,3-beta-mannosyl-terminus ?
UDP-N-acetyl-D-glucosamine + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R absolute specificity for terminal branched mannosyl residues Sus scrofa UDP + alpha-D-mannosyl-1,6-(alpha-D-mannosyl-1,3)-alpha-D-mannosyl-1,6-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3)-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R transfers an N-acetylglucosamine in beta-1,2-linkage to mannosyl-1,3-beta-mannosyl-terminus ?
UDP-N-acetyl-D-glucosamine + glycopeptides prepared from porcine IgC
-
 Sus scrofa ?
-
 ?
UDP-N-acetyl-D-glucosamine + ovalbumin substrate: glycopeptides prepared from ovalbumin Sus scrofa ?
-
 ?