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Literature summary for 2.4.1.10 extracted from

  • Li, W.; Yu, S.; Zhang, T.; Jiang, B.; Mu, W.
    Synthesis of raffinose by transfructosylation using recombinant levansucrase from Clostridium arbusti SL206 (2017), J. Sci. Food. Agric., 97, 43-49 .
    View publication on PubMed
Search for more literature for 2.4.1.10

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Clostridium arbusti

Organism

Organism UniProt Comment Textmining
Clostridium arbusti
-
-
-
Clostridium arbusti SL206
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + H2O
-
 Clostridium arbusti D-glucose + D-fructose
-
 ?
sucrose + H2O
-
 Clostridium arbusti SL206 D-glucose + D-fructose
-
 ?
sucrose + melibiose when 30% (w/v) sucrose and 30% (w/v) melibiose are catalyzed using 3% (w/v) whole cells at pH 6.5 (50 mM sodium phosphate buffer) and 55°C, the highest raffinose yield is 222 g/l after a 6 h reaction. The conversion ratio from each substrate to raffinose is 50% Clostridium arbusti D-glucose + raffinose
-
 ?
sucrose + melibiose when 30% (w/v) sucrose and 30% (w/v) melibiose are catalyzed using 3% (w/v) whole cells at pH 6.5 (50 mM sodium phosphate buffer) and 55°C, the highest raffinose yield is 222 g/l after a 6 h reaction. The conversion ratio from each substrate to raffinose is 50% Clostridium arbusti SL206 D-glucose + raffinose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Clostridium arbusti

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 in 50 mM sodium phosphate buffer Clostridium arbusti