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Literature summary for 2.4.1.10 extracted from

  • Ko, H.; Bae, J.H.; Sung, B.H.; Kim, M.J.; Kim, C.H.; Oh, B.R.; Sohn, J.H.
    Efficient production of levan using a recombinant yeast Saccharomyces cerevisiae hypersecreting a bacterial levansucrase (2019), J. Ind. Microbiol. Biotechnol., 46, 1611-1620 .
    View publication on PubMed
Search for more literature for 2.4.1.10

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain Y2805gs Rahnella aquatilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside Rahnella aquatilis
-
 D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
 ?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside Rahnella aquatilis ATCC 33071
-
 D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
 ?

Organism

Organism UniProt Comment Textmining
Rahnella aquatilis O54435
-
-
Rahnella aquatilis ATCC 33071 O54435
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA affinity column chromatography Rahnella aquatilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
101.2
-
 crude enzyme, at pH 6.0 and 37°C Rahnella aquatilis
195.3
-
 after 1.93fold purification, at pH 6.0 and 37°C Rahnella aquatilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + sucrose the recombinant enzyme shows 80% conversion yield Rahnella aquatilis D-glucose + levan
-
 ?
sucrose + sucrose the recombinant enzyme shows 80% conversion yield Rahnella aquatilis ATCC 33071 D-glucose + levan
-
 ?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
-
 Rahnella aquatilis D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
 ?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
-
 Rahnella aquatilis ATCC 33071 D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
 ?

Subunits

Subunits Comment Organism
? x * 47000, SDS-PAGE Rahnella aquatilis

Synonyms

Synonyms Comment Organism
LscrA
-
 Rahnella aquatilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Rahnella aquatilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 about 88% activity 20°C, about 100% activity at 30-40°C, about 30% activity 50°C Rahnella aquatilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Rahnella aquatilis

pH Range

pH Minimum pH Maximum Comment Organism
5 7 about 85% activity at pH 5.0, 100% activity at pH 6.0, about 70% activity at pH 7.0 Rahnella aquatilis