Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.10 extracted from

  • Salama, B.M.; Helmy, W.A.; Ragab, T.I.M.; Ali, M.M.; Taie, H.A.A.; Esawy, M.A.
    Characterization of a new efficient low molecular weight Bacillus subtilis NRC 16 levansucrase and its levan (2019), J. Basic Microbiol., 59, 1004-1015 .
    View publication on PubMed
Search for more literature for 2.4.1.10

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 37.9% residual activity at 0.02 mM Bacillus subtilis
Ca2+ 46.1% residual activity at 0.02 mM Bacillus subtilis
Cr3+ 42.4% residual activity at 0.02 mM Bacillus subtilis
Cu2+ 52.2% residual activity at 0.02 mM Bacillus subtilis
EDTA 41.8% residual activity at 0.02 mM Bacillus subtilis
K+ 49.1% residual activity at 0.02 mM Bacillus subtilis
Mg2+ 45.4% residual activity at 0.02 mM Bacillus subtilis
Na+ 54.5% residual activity at 0.02 mM Bacillus subtilis
Zn2+ 33.9% residual activity at 0.02 mM Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside Bacillus subtilis
-
 D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
 ?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside Bacillus subtilis NRC 16
-
 D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis NRC 16
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + sucrose
-
 Bacillus subtilis D-glucose + levan
-
 ?
sucrose + sucrose
-
 Bacillus subtilis NRC 16 D-glucose + levan
-
 ?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
-
 Bacillus subtilis D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
 ?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
-
 Bacillus subtilis NRC 16 D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
 ?

Subunits

Subunits Comment Organism
? x * 14000, SDS-PAGE Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Bacillus subtilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 55 the enzyme is active between 37 and 55°C Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35 50 the enzyme is stable at temperatures (35°C and 37°C) in all incubation periods. A decrease in the enzyme activity is noticed at 40°C and 45°C after 2 h 12% and 19%, respectively. At 50°C there is about 40% loss in activity after 2 h Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
 Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
4.2 9.2 26% and 18% decrease in levansucrase activity is noticed at pH 4.2 and 9.2, respectively Bacillus subtilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.2 9.2 the enzyme is stable at pH 5.2-9.2. At pH 4.2 the activity decreases gradually to 85% after 2 h Bacillus subtilis