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Literature summary for 2.4.1.10 extracted from

  • Polsinelli, I.; Caliandro, R.; Demitri, N.; Benini, S.
    The structure of sucrose-soaked levansucrase crystals from Erwinia tasmaniensis reveals a binding pocket for levanbiose (2020), Int. J. Mol. Sci., 21, 83 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.10

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) star pLysS cells Erwinia tasmaniensis

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with levanbiose, hanging drop vapor diffusion method, using 28% (v/v) glycerol, 14% (w/v) PEG4000, 2.5 mM manganese(II) chloride tetrahydrate, 2.5 mM cobalt(II) chloride hexahydrate, 2.5 mM nickel(II) chloride hexahydrate, and 2.5 mM zinc acetate dihydrate Erwinia tasmaniensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside Erwinia tasmaniensis
-
 D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
 ?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside Erwinia tasmaniensis Et1/99
-
 D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
 ?

Organism

Organism UniProt Comment Textmining
Erwinia tasmaniensis B2VCC3
-
-
Erwinia tasmaniensis Et1/99 B2VCC3
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Erwinia tasmaniensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme also hydrolyzes sucrose to D-glucose and D-fructose Erwinia tasmaniensis ?
-
-
additional information the enzyme also hydrolyzes sucrose to D-glucose and D-fructose Erwinia tasmaniensis Et1/99 ?
-
-
sucrose + sucrose
-
 Erwinia tasmaniensis D-glucose + low-molecular weight levan + short-chain fructooligosaccharides (DP 3-6)
-
 ?
sucrose + sucrose
-
 Erwinia tasmaniensis Et1/99 D-glucose + low-molecular weight levan + short-chain fructooligosaccharides (DP 3-6)
-
 ?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
-
 Erwinia tasmaniensis D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
 ?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
-
 Erwinia tasmaniensis Et1/99 D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
 ?

Synonyms

Synonyms Comment Organism
Lsc
-
 Erwinia tasmaniensis