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Literature summary for 2.4.1.10 extracted from

  • Polsinelli, I.; Caliandro, R.; Salomone-Stagni, M.; Demitri, N.; Rejzek, M.; Field, R.A.; Benini, S.
    Comparison of the levansucrase from the epiphyte Erwinia tasmaniensis vs its homologue from the phytopathogen Erwinia amylovora (2019), Int. J. Biol. Macromol., 127, 496-501 .
    View publication on PubMed
Search for more literature for 2.4.1.10

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) Star pLysS cells Erwinia tasmaniensis

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 28% (v/v) glycerol, 14% (w/v) PEG4000, 2.5 mM manganese(II) chloride tetrahydrate, 2.5 mM cobalt(II) chloride hexahydrate, 2.5 mM nickel(II) chloride hexahydrate, 2.5 mM zinc acetate dihydrate Erwinia tasmaniensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
33.6
-
 sucrose at pH 6.5 and 37°C Erwinia amylovora
50.5
-
 sucrose at pH 6.5 and 37°C Erwinia tasmaniensis

Organism

Organism UniProt Comment Textmining
Erwinia amylovora
-
-
-
Erwinia tasmaniensis B2VCC3
-
-
Erwinia tasmaniensis Et1/99 B2VCC3
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex S200 gel filtration Erwinia tasmaniensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + sucrose
-
 Erwinia amylovora D-glucose + 6-kestose + 6-nystose + 6,6,6-kestopentaose + levanbiose + levantriose + ?
-
 ?
sucrose + sucrose
-
 Erwinia tasmaniensis D-glucose + 6-kestose + 6-nystose + 6,6,6-kestopentaose + levanbiose + levantriose + ?
-
 ?
sucrose + sucrose
-
 Erwinia tasmaniensis Et1/99 D-glucose + 6-kestose + 6-nystose + 6,6,6-kestopentaose + levanbiose + levantriose + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 46430, calculated from amino acid sequence Erwinia tasmaniensis
? x * 46520, calculated from amino acid sequence Erwinia amylovora

Synonyms

Synonyms Comment Organism
Lsc
-
 Erwinia amylovora
Lsc
-
 Erwinia tasmaniensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Erwinia amylovora
37
-
-
 Erwinia tasmaniensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 42 the highest activity is observed in the range 37-42°C Erwinia tasmaniensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
497.9
-
 sucrose at pH 6.5 and 37°C Erwinia tasmaniensis
502.7
-
 sucrose at pH 6.5 and 37°C Erwinia amylovora

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Erwinia amylovora
6.5
-
-
 Erwinia tasmaniensis

pH Range

pH Minimum pH Maximum Comment Organism
5.5 6.5 the best pH range for activity is between 5.5 and 6.5. Above pH 8.0 the enzyme shows an almost complete inhibition Erwinia tasmaniensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
607
-
 sucrose at pH 6.5 and 37°C Erwinia tasmaniensis
897
-
 sucrose at pH 6.5 and 37°C Erwinia amylovora