Literature summary for 2.4.1.10 extracted from

Mostafa, F.A.; Abdel Wahab, W.A.; Salah, H.A.; Nawwar, G.A.M.; Esawy, M.A.
Kinetic and thermodynamic characteristic of Aspergillus awamori EM66 levansucrase (2018), Int. J. Biol. Macromol., 119, 232-239 .

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Activating Compound

Activating Compound	Comment	Organism	Structure
NaCl	maximum activity at 3.0 M	Aspergillus awamori

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside	Aspergillus awamori	-	D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside	-	?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside	Aspergillus awamori EM66	-	D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside	-	?

Organism

Organism	UniProt	Comment	Textmining
Aspergillus awamori	-	-	-
Aspergillus awamori EM66	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
partial purification by acetone precipitation. Full purification by acetone precipitation and DEAE-Sepharose column chromatography	Aspergillus awamori

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2535	-	crude enzyme, at pH 5.2 and 30°C	Aspergillus awamori
10590	-	partially (4.178fold) purified enzyme, at pH 5.2 and 30°C	Aspergillus awamori
18511	-	7.3fold purified enzyme, at pH 5.2 and 30°C	Aspergillus awamori

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sucrose + sucrose	-	Aspergillus awamori	D-glucose + levan	-	?
sucrose + sucrose	-	Aspergillus awamori EM66	D-glucose + levan	-	?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside	-	Aspergillus awamori	D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside	-	?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside	-	Aspergillus awamori EM66	D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside	-	?

Subunits

Subunits	Comment	Organism
?	x * 44500, SDS-PAGE	Aspergillus awamori

Synonyms

Synonyms	Comment	Organism
fructansucrase	-	Aspergillus awamori

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Aspergillus awamori

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
45	55	he partially purified enzyme form retains about 76% of its original activity after 1 h at 55°C while the purified form retains about 57% after 45 min. The half-life of the partially purified form is 3286, 1150, and 718 min at 45, 50 and 55°C, respectively. The half-life of the fully purified form is 575, 319 and 176 min at 45, 50 and 55°C, respectively	Aspergillus awamori

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Release 2023.1 (January 2023)