Literature summary for 2.4.1.10 extracted from

Hill, A.; Chen, L.; Mariage, A.; Petit, J.; De Berardinis, V.; Karboune, S.
Discovery of new levansucrase enzymes with interesting properties and improved catalytic activity to produce levan and fructooligosaccharides (2019), Catal. Sci. Technol., 9, 2931-2944 .

No PubMed abstract available

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Leuconostoc mesenteroides
expressed in Escherichia coli BL21(DE3) cells	Paraburkholderia graminis
expressed in Escherichia coli BL21(DE3) cells	Novosphingobium aromaticivorans
expressed in Escherichia coli BL21(DE3) cells	Streptococcus salivarius
expressed in Escherichia coli BL21(DE3) cells	Vibrio natriegens
expressed in Escherichia coli BL21(DE3) cells	Natronococcus amylolyticus
expressed in Escherichia coli BL21(DE3) cells	Beijerinckia indica subsp. indica
expressed in Escherichia coli BL21(DE3) cells	Gluconobacter oxydans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
2	-	sucrose	hydrolytic activity, at pH 6.0 and 37°C	Vibrio natriegens
2	3	sucrose	total activity, at pH 6.0 and 37°C	Beijerinckia indica subsp. indica
3	-	sucrose	total activity, at pH 6.0 and 37°C	Vibrio natriegens
4	-	sucrose	hydrolytic activity, at pH 6.0 and 37°C	Novosphingobium aromaticivorans
8	-	sucrose	total activity, at pH 6.0 and 37°C	Novosphingobium aromaticivorans
8	-	sucrose	transfructosylating activity, at pH 6.0 and 37°C	Streptococcus salivarius
9	-	sucrose	hydrolytic activity, at pH 6.0 and 37°C	Streptococcus salivarius
9	-	sucrose	total activity, at pH 6.0 and 37°C	Streptococcus salivarius
20	-	sucrose	hydrolytic activity, at pH 6.0 and 37°C	Paraburkholderia graminis
21	-	sucrose	hydrolytic activity, at pH 6.0 and 37°C	Beijerinckia indica subsp. indica
30	-	sucrose	total activity, at pH 6.0 and 37°C	Paraburkholderia graminis
436	-	sucrose	transfructosylating activity, at pH 6.0 and 37°C	Vibrio natriegens
440	-	sucrose	transfructosylating activity, at pH 6.0 and 37°C	Leuconostoc mesenteroides
467	-	sucrose	transfructosylating activity, at pH 6.0 and 37°C	Beijerinckia indica subsp. indica
479	-	sucrose	transfructosylating activity, at pH 6.0 and 37°C	Paraburkholderia graminis
480	-	sucrose	total activity, at pH 6.0 and 37°C	Gluconobacter oxydans
519	-	sucrose	transfructosylating activity, at pH 6.0 and 37°C	Novosphingobium aromaticivorans
617	-	sucrose	hydrolytic activity, at pH 6.0 and 37°C	Gluconobacter oxydans
674	-	sucrose	transfructosylating activity, at pH 6.0 and 37°C	Gluconobacter oxydans

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside	Leuconostoc mesenteroides	-	D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside	-	?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside	Paraburkholderia graminis	-	D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside	-	?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside	Novosphingobium aromaticivorans	-	D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside	-	?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside	Streptococcus salivarius	-	D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside	-	?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside	Vibrio natriegens	-	D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside	-	?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside	Natronococcus amylolyticus	-	D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside	-	?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside	Beijerinckia indica subsp. indica	-	D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside	-	?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside	Gluconobacter oxydans	-	D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside	-	?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside	Beijerinckia indica subsp. indica ATCC 9039	-	D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside	-	?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside	Gluconobacter oxydans 621H	-	D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside	-	?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside	Natronococcus amylolyticus DSM 10524	-	D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside	-	?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside	Streptococcus salivarius K12	-	D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside	-	?

Organism

Organism	UniProt	Comment	Textmining
Beijerinckia indica subsp. indica	B2IF78	-	-
Beijerinckia indica subsp. indica ATCC 9039	B2IF78	-	-
Gluconobacter oxydans	Q5FSK0	-	-
Gluconobacter oxydans 621H	Q5FSK0	-	-
Leuconostoc mesenteroides	Q1L7R6	-	-
Natronococcus amylolyticus	L9X0M4	-	-
Natronococcus amylolyticus DSM 10524	L9X0M4	-	-
Novosphingobium aromaticivorans	Q2G754	-	-
Paraburkholderia graminis	B1G3X6	-	-
Streptococcus salivarius	J7TH23	-	-
Streptococcus salivarius K12	J7TH23	-	-
Vibrio natriegens	A0A1B1EI54	-	-

Purification (Commentary)

Purification (Comment)	Organism
HisTrap column chromatography	Leuconostoc mesenteroides
HisTrap column chromatography	Paraburkholderia graminis
HisTrap column chromatography	Novosphingobium aromaticivorans
HisTrap column chromatography	Streptococcus salivarius
HisTrap column chromatography	Vibrio natriegens
HisTrap column chromatography	Natronococcus amylolyticus
HisTrap column chromatography	Beijerinckia indica subsp. indica
HisTrap column chromatography	Gluconobacter oxydans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
additional information	the enzyme also hydrolyzes sucrose to D-glucose and D-fructose	Novosphingobium aromaticivorans	?	-	-
additional information	the enzyme also hydrolyzes sucrose to D-glucose and D-fructose	Streptococcus salivarius	?	-	-
additional information	the enzyme also hydrolyzes sucrose to D-glucose and D-fructose	Vibrio natriegens	?	-	-
additional information	the enzyme also hydrolyzes sucrose to D-glucose and D-fructose	Natronococcus amylolyticus	?	-	-
additional information	the enzyme also hydrolyzes sucrose to D-glucose and D-fructose	Beijerinckia indica subsp. indica	?	-	-
additional information	the enzyme also hydrolyzes sucrose to D-glucose and D-fructose	Gluconobacter oxydans	?	-	-
additional information	the enzyme also shows hydrolytic activity with sucrose producing D-glucose and D-fructose	Paraburkholderia graminis	?	-	-
additional information	the enzyme also hydrolyzes sucrose to D-glucose and D-fructose	Beijerinckia indica subsp. indica ATCC 9039	?	-	-
additional information	the enzyme also hydrolyzes sucrose to D-glucose and D-fructose	Gluconobacter oxydans 621H	?	-	-
additional information	the enzyme also hydrolyzes sucrose to D-glucose and D-fructose	Natronococcus amylolyticus DSM 10524	?	-	-
additional information	the enzyme also hydrolyzes sucrose to D-glucose and D-fructose	Streptococcus salivarius K12	?	-	-
sucrose + sucrose	-	Leuconostoc mesenteroides	D-glucose + levan	-	?
sucrose + sucrose	-	Paraburkholderia graminis	D-glucose + levan	-	?
sucrose + sucrose	-	Novosphingobium aromaticivorans	D-glucose + levan	-	?
sucrose + sucrose	-	Streptococcus salivarius	D-glucose + levan	-	?
sucrose + sucrose	-	Vibrio natriegens	D-glucose + levan	-	?
sucrose + sucrose	-	Natronococcus amylolyticus	D-glucose + levan	-	?
sucrose + sucrose	-	Beijerinckia indica subsp. indica	D-glucose + levan	-	?
sucrose + sucrose	-	Gluconobacter oxydans	D-glucose + levan	-	?
sucrose + sucrose	-	Beijerinckia indica subsp. indica ATCC 9039	D-glucose + levan	-	?
sucrose + sucrose	-	Gluconobacter oxydans 621H	D-glucose + levan	-	?
sucrose + sucrose	-	Natronococcus amylolyticus DSM 10524	D-glucose + levan	-	?
sucrose + sucrose	-	Streptococcus salivarius K12	D-glucose + levan	-	?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside	-	Leuconostoc mesenteroides	D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside	-	?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside	-	Paraburkholderia graminis	D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside	-	?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside	-	Novosphingobium aromaticivorans	D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside	-	?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside	-	Streptococcus salivarius	D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside	-	?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside	-	Vibrio natriegens	D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside	-	?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside	-	Natronococcus amylolyticus	D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside	-	?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside	-	Beijerinckia indica subsp. indica	D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside	-	?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside	-	Gluconobacter oxydans	D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside	-	?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside	-	Beijerinckia indica subsp. indica ATCC 9039	D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside	-	?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside	-	Gluconobacter oxydans 621H	D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside	-	?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside	-	Natronococcus amylolyticus DSM 10524	D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside	-	?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside	-	Streptococcus salivarius K12	D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside	-	?

Subunits

Subunits	Comment	Organism
?	x * 46800, calculated from amino acid sequence	Vibrio natriegens
?	x * 47900, calculated from amino acid sequence	Gluconobacter oxydans
?	x * 113400, calculated from amino acid sequence	Leuconostoc mesenteroides
?	x * 47400, calculated from amino acid sequence	Paraburkholderia graminis
?	x * 58000, calculated from amino acid sequence	Beijerinckia indica subsp. indica
?	x * 104200, calculated from amino acid sequence	Streptococcus salivarius
?	x * 41500, calculated from amino acid sequence	Novosphingobium aromaticivorans
?	x * 51400, calculated from amino acid sequence	Natronococcus amylolyticus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	-	the enzyme has a half-life of 11.8 min at 50°C	Natronococcus amylolyticus
50	-	the enzyme has a half-life of 20.1 min at 50°C	Streptococcus salivarius
50	-	the enzyme has a half-life of 24.3 min at 50°C	Novosphingobium aromaticivorans
50	-	the enzyme has a half-life of 290.7 min at 50°C	Paraburkholderia graminis
50	-	the enzyme has a half-life of 39.4 min at 50°C	Vibrio natriegens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism
1500	-	sucrose	transfructosylating activity, at pH 6.0 and 37°C	Leuconostoc mesenteroides
4700	-	sucrose	transfructosylating activity, at pH 6.0 and 37°C	Paraburkholderia graminis
5700	-	sucrose	hydrolytic activity, at pH 6.0 and 37°C	Paraburkholderia graminis
7200	-	sucrose	total activity, at pH 6.0 and 37°C	Paraburkholderia graminis
8000	-	sucrose	hydrolytic activity, at pH 6.0 and 37°C	Beijerinckia indica subsp. indica
9000	-	sucrose	transfructosylating activity, at pH 6.0 and 37°C	Beijerinckia indica subsp. indica
12000	-	sucrose	total activity, at pH 6.0 and 37°C	Beijerinckia indica subsp. indica
19300	-	sucrose	hydrolytic activity, at pH 6.0 and 37°C	Streptococcus salivarius
33600	-	sucrose	transfructosylating activity, at pH 6.0 and 37°C	Streptococcus salivarius
55400	-	sucrose	total activity, at pH 6.0 and 37°C	Streptococcus salivarius
100000	-	sucrose	transfructosylating activity, at pH 6.0 and 37°C	Vibrio natriegens
157300	-	sucrose	transfructosylating activity, at pH 6.0 and 37°C	Novosphingobium aromaticivorans
176000	-	sucrose	hydrolytic activity, at pH 6.0 and 37°C	Novosphingobium aromaticivorans
200000	-	sucrose	hydrolytic activity, at pH 6.0 and 37°C	Vibrio natriegens
200000	-	sucrose	total activity, at pH 6.0 and 37°C	Vibrio natriegens
300000	-	sucrose	hydrolytic activity, at pH 6.0 and 37°C	Gluconobacter oxydans
300000	-	sucrose	transfructosylating activity, at pH 6.0 and 37°C	Gluconobacter oxydans
317400	-	sucrose	total activity, at pH 6.0 and 37°C	Novosphingobium aromaticivorans
600000	-	sucrose	total activity, at pH 6.0 and 37°C	Gluconobacter oxydans

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism
3.47	-	sucrose	transfructosylating activity, at pH 6.0 and 37°C	Leuconostoc mesenteroides
9.87	-	sucrose	transfructosylating activity, at pH 6.0 and 37°C	Paraburkholderia graminis
20	-	sucrose	transfructosylating activity, at pH 6.0 and 37°C	Beijerinckia indica subsp. indica
246	-	sucrose	total activity, at pH 6.0 and 37°C	Paraburkholderia graminis
289	-	sucrose	hydrolytic activity, at pH 6.0 and 37°C	Paraburkholderia graminis
303	-	sucrose	transfructosylating activity, at pH 6.0 and 37°C	Novosphingobium aromaticivorans
350	-	sucrose	transfructosylating activity, at pH 6.0 and 37°C	Vibrio natriegens
382	-	sucrose	hydrolytic activity, at pH 6.0 and 37°C	Beijerinckia indica subsp. indica
493	-	sucrose	transfructosylating activity, at pH 6.0 and 37°C	Gluconobacter oxydans
534	-	sucrose	total activity, at pH 6.0 and 37°C	Beijerinckia indica subsp. indica
555	-	sucrose	hydrolytic activity, at pH 6.0 and 37°C	Gluconobacter oxydans
1192	-	sucrose	total activity, at pH 6.0 and 37°C	Gluconobacter oxydans
2256	-	sucrose	hydrolytic activity, at pH 6.0 and 37°C	Streptococcus salivarius
4058	-	sucrose	transfructosylating activity, at pH 6.0 and 37°C	Streptococcus salivarius
6124	-	sucrose	total activity, at pH 6.0 and 37°C	Streptococcus salivarius
32461	-	sucrose	total activity, at pH 6.0 and 37°C	Novosphingobium aromaticivorans
40735	-	sucrose	hydrolytic activity, at pH 6.0 and 37°C	Novosphingobium aromaticivorans
79574	-	sucrose	total activity, at pH 6.0 and 37°C	Vibrio natriegens
100555	-	sucrose	hydrolytic activity, at pH 6.0 and 37°C	Vibrio natriegens