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Literature summary for 2.4.1.10 extracted from

  • Phengnoi, P.; Charoenwongpaiboon, T.; Wangpaiboon, K.; Klaewkla, M.; Nakapong, S.; Visessanguan, W.; Ito, K.; Pichyangkura, R.; Kuttiyawong, K.
    Levansucrase from Bacillus amyloliquefaciens KK9 and its Y237S variant producing the high bioactive levan-type fructooligosaccharides (2020), Biomolecules, 10, 692 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.10

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Star(DE3) cells Bacillus amyloliquefaciens

Protein Variants

Protein Variants Comment Organism
Y237S the mutant efficiently produces a higher amount of short-chain levan-typed fructooligosaccharide than wild type with increased prebiotic activity Bacillus amyloliquefaciens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside Bacillus amyloliquefaciens
-
 D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
 ?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside Bacillus amyloliquefaciens KK9
-
 D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus amyloliquefaciens M9TNM8
-
-
Bacillus amyloliquefaciens KK9 M9TNM8
-
-

Purification (Commentary)

Purification (Comment) Organism
CM Toyopearl column chromatography Bacillus amyloliquefaciens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
81.9
-
 crude enzyme, at pH 6.0 and 37°C Bacillus amyloliquefaciens
152.4
-
 after 1.9fold purification, at pH 6.0 and 37°C Bacillus amyloliquefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + sucrose
-
 Bacillus amyloliquefaciens D-glucose + levan type-fructan
-
 ?
sucrose + sucrose
-
 Bacillus amyloliquefaciens KK9 D-glucose + levan type-fructan
-
 ?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
-
 Bacillus amyloliquefaciens D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
 ?
sucrose + [beta-D-fructofuranosyl-(2->6)]n alpha-D-glucopyranoside
-
 Bacillus amyloliquefaciens KK9 D-glucose + [beta-D-fructofuranosyl-(2->6)]n+1 alpha-D-glucopyranoside
-
 ?

Subunits

Subunits Comment Organism
? x * 53000, SDS-PAGE Bacillus amyloliquefaciens
? x * 5297, calculated from amino acid sequence Bacillus amyloliquefaciens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Bacillus amyloliquefaciens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 more than 50% activity between 20 and 50°C Bacillus amyloliquefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Bacillus amyloliquefaciens

pH Range

pH Minimum pH Maximum Comment Organism
5 7 more than 60% activity between pH 5.0 and 7.0 Bacillus amyloliquefaciens